Detailed information of TA system TA135037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 250610..251145
• Replicon: plasmid pColV-PU1
• Accession: NZ_CP042245
• Organism: Escherichia coli strain PU-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: FQU83_RS01525
• Protein ID: WP_000222760.1
• Coordinates: 250610..250897 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: FQU83_RS01530
• Protein ID: WP_001132900.1
• Coordinates: 250894..251145 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU83_RS01495	246193..247414	-	1222	Protein_259	ISL3 family transposase	-
FQU83_RS01500	247514..247878	+	365	Protein_260	IS1 family transposase	-
FQU83_RS01505	247933..248715	-	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
FQU83_RS01510	248712..249734	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
FQU83_RS01515	249823..250170	+	348	Protein_263	IS1-like element IS1A family transposase	-
FQU83_RS01525	250610..250897	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FQU83_RS01530	250894..251145	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FQU83_RS01535	252040..253791	-	1752	Protein_266	Tn3-like element TnAs1 family transposase	-
FQU83_RS01540	253780..254187	+	408	WP_001214976.1	cysteine hydrolase	-
FQU83_RS01545	254325..255209	+	885	WP_000058717.1	EamA family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..285922	285922
	-	inside	IScluster/Tn	tet(A)	-	246455..261058	14603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T135037 WP_000222760.1 NZ_CP042245:c250897-250610 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T135037 NZ_CP042245:c250897-250610 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT135037 WP_001132900.1 NZ_CP042245:c251145-250894 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT135037 NZ_CP042245:c251145-250894 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2