Detailed information of TA system TA135036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 193757..194133
• Replicon: plasmid pColV-PU1
• Accession: NZ_CP042245
• Organism: Escherichia coli strain PU-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: FQU83_RS01175
• Protein ID: WP_000969988.1
• Coordinates: 193757..193939 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: FQU83_RS01180
• Protein ID: WP_001324224.1
• Coordinates: 193936..194133 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU83_RS01160	191756..192790	-	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-
FQU83_RS01170	193350..193658	-	309	WP_000377483.1	hypothetical protein	-
FQU83_RS01175	193757..193939	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FQU83_RS01180	193936..194133	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
FQU83_RS01185	194815..196089	+	1275	WP_000489608.1	HlyD family secretion protein	-
FQU83_RS01190	196064..198178	+	2115	WP_001183603.1	peptidase domain-containing ABC transporter	-
FQU83_RS01195	198348..198659	-	312	WP_001259758.1	colicin V	-
FQU83_RS01200	198637..198873	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..285922	285922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T135036 WP_000969988.1 NZ_CP042245:c193939-193757 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T135036 NZ_CP042245:c193939-193757 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT135036 WP_001324224.1 NZ_CP042245:c194133-193936 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT135036 NZ_CP042245:c194133-193936 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3