Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 117428..117953 Replicon plasmid pColV-PU1
Accession NZ_CP042245
Organism Escherichia coli strain PU-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FQU83_RS00795 Protein ID WP_001159871.1
Coordinates 117428..117733 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag FQU83_RS00800 Protein ID WP_000813630.1
Coordinates 117735..117953 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQU83_RS00780 113384..114589 - 1206 WP_001442122.1 AAA family ATPase -
FQU83_RS00785 115210..115941 + 732 WP_000504262.1 replication initiation protein -
FQU83_RS00790 116621..117427 - 807 WP_021543129.1 site-specific integrase -
FQU83_RS00795 117428..117733 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FQU83_RS00800 117735..117953 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FQU83_RS00810 118519..119031 + 513 WP_000151784.1 hypothetical protein -
FQU83_RS00815 119065..120198 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
FQU83_RS00820 120365..121138 - 774 WP_000905949.1 hypothetical protein -
FQU83_RS00825 121151..121651 - 501 WP_000528932.1 hypothetical protein -
FQU83_RS00830 121916..122146 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FQU83_RS00835 122143..122559 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..285922 285922


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T135033 WP_001159871.1 NZ_CP042245:c117733-117428 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T135033 NZ_CP042245:c117733-117428 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT135033 WP_000813630.1 NZ_CP042245:c117953-117735 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT135033 NZ_CP042245:c117953-117735 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References