Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 61971..62518 Replicon plasmid pColV-PU1
Accession NZ_CP042245
Organism Escherichia coli strain PU-1

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag FQU83_RS00440 Protein ID WP_000969996.1
Coordinates 61971..62252 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag FQU83_RS00445 Protein ID WP_000079928.1
Coordinates 62249..62518 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQU83_RS00415 58456..59579 + 1124 WP_201789284.1 IS3 family transposase -
FQU83_RS00420 59686..60489 + 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
FQU83_RS00425 60489..61325 + 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
FQU83_RS00435 61539..61886 - 348 WP_001115718.1 hypothetical protein -
FQU83_RS00440 61971..62252 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FQU83_RS00445 62249..62518 - 270 WP_000079928.1 hypothetical protein Antitoxin
FQU83_RS00460 63432..64289 - 858 WP_000130961.1 incFII family plasmid replication initiator RepA -
FQU83_RS00465 64282..64356 - 75 WP_001375168.1 RepA leader peptide Tap -
FQU83_RS00475 64580..64840 - 261 WP_000083819.1 replication regulatory protein RepA -
FQU83_RS00480 65079..65669 - 591 WP_021573171.1 DUF2726 domain-containing protein -
FQU83_RS00485 65708..65917 - 210 WP_021573170.1 hemolysin expression modulator Hha -
FQU83_RS00490 65963..66424 - 462 WP_000978818.1 thermonuclease family protein -
FQU83_RS00495 66670..66882 - 213 WP_001299730.1 ANR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..285922 285922


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T135028 WP_000969996.1 NZ_CP042245:c62252-61971 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T135028 NZ_CP042245:c62252-61971 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT135028 WP_000079928.1 NZ_CP042245:c62518-62249 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT135028 NZ_CP042245:c62518-62249 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGATCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCATCTGCTGCAGCATGGAGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References