Detailed information of TA system TA135022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 680597..681224
• Replicon: chromosome
• Accession: NZ_CP042243
• Organism: Crassaminicella sp. SY095

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FQB35_RS03120
• Protein ID: WP_148808587.1
• Coordinates: 680874..681224 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FQB35_RS03115
• Protein ID: WP_148808586.1
• Coordinates: 680597..680872 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQB35_RS03090	675893..676489	+	597	WP_168198220.1	hypothetical protein	-
FQB35_RS03095	676582..676950	+	369	WP_148810744.1	holo-ACP synthase	-
FQB35_RS03100	676977..678545	+	1569	WP_148808580.1	NAD(P)H-hydrate dehydratase	-
FQB35_RS03105	678602..679219	+	618	WP_207707335.1	outer-membrane lipoprotein carrier protein LolA	-
FQB35_RS03110	679259..680443	+	1185	WP_148808584.1	alanine racemase	-
FQB35_RS03115	680597..680872	+	276	WP_148808586.1	ribbon-helix-helix protein, CopG family	Antitoxin
FQB35_RS03120	680874..681224	+	351	WP_148808587.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FQB35_RS03125	681498..682142	+	645	WP_148808589.1	DNA alkylation repair protein	-
FQB35_RS03130	682265..685072	+	2808	WP_148808590.1	phosphodiester glycosidase family protein	-
FQB35_RS03135	685141..685626	+	486	WP_148808592.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12789.82 Da
• Isoelectric Point: 6.4714

>T135022 WP_148808587.1 NZ_CP042243:680874-681224 [Crassaminicella sp. SY095]
VIVKRGDIFYADLSPVVGSEQGGVRPVLVVQNDIGNKYSPTVIVAAITSQINKAKLPTHIEISASEYGLPKDSVVLLEQI
RTIDKKRLREKIGHSDEEMMNKVNEALLISFGLIDF

Nucleotide

• Length: 351 bp

>T135022 NZ_CP042243:680874-681224 [Crassaminicella sp. SY095]
GTGATTGTAAAAAGAGGAGACATATTTTATGCAGATCTTAGTCCTGTTGTTGGTTCTGAGCAAGGTGGTGTAAGACCGGT
CCTTGTTGTTCAGAATGATATAGGAAATAAATATAGTCCTACGGTTATTGTGGCAGCAATCACCTCTCAGATTAATAAAG
CAAAGCTACCAACGCATATTGAGATTAGCGCCTCAGAATATGGGTTACCGAAGGACTCAGTGGTGTTATTAGAGCAGATT
CGAACTATTGATAAAAAGAGACTAAGAGAAAAAATAGGGCATTCTGATGAAGAAATGATGAATAAAGTAAATGAAGCTTT
ATTAATTAGCTTTGGATTAATAGATTTTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10551.36 Da
• Isoelectric Point: 8.9483

>AT135022 WP_148808586.1 NZ_CP042243:680597-680872 [Crassaminicella sp. SY095]
MAESKRIMISLPNTLLQEVDNIVAMEKGNRSQFIREAMRLYIRERRKIQIRENMKKGYREMGAMNLALSEVGLDLDISAL
KSYEAKLAECE

Nucleotide

• Length: 276 bp

>AT135022 NZ_CP042243:680597-680872 [Crassaminicella sp. SY095]
ATGGCTGAGTCCAAACGAATTATGATTAGTTTACCAAACACACTTTTACAAGAAGTAGATAATATTGTTGCAATGGAGAA
AGGAAACAGAAGCCAATTTATTAGAGAAGCGATGAGATTGTATATTCGTGAAAGGCGTAAGATTCAAATAAGAGAAAATA
TGAAAAAGGGTTATAGAGAAATGGGGGCTATGAATTTAGCATTATCAGAGGTAGGATTAGATTTAGATATAAGTGCACTA
AAGAGCTATGAAGCAAAGTTAGCGGAGTGTGAGTAG