Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3760206..3760732 Replicon chromosome
Accession NZ_CP042220
Organism Dickeya poaceiphila strain NCPPB 569

Toxin (Protein)


Gene name ccdB Uniprot ID A0A5B8IAT4
Locus tag Dpoa569_RS16920 Protein ID WP_042868396.1
Coordinates 3760427..3760732 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A5B8IBF7
Locus tag Dpoa569_RS16915 Protein ID WP_038903500.1
Coordinates 3760206..3760424 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Dpoa569_RS16885 3755479..3756054 + 576 WP_042868404.1 transcriptional regulator -
Dpoa569_RS16895 3756444..3757702 + 1259 Protein_3284 integrase arm-type DNA-binding domain-containing protein -
Dpoa569_RS16900 3757842..3758030 - 189 WP_128569670.1 hypothetical protein -
Dpoa569_RS16905 3758153..3758503 + 351 WP_042868401.1 hypothetical protein -
Dpoa569_RS16910 3759404..3760147 + 744 WP_042868399.1 hypothetical protein -
Dpoa569_RS16915 3760206..3760424 + 219 WP_038903500.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
Dpoa569_RS16920 3760427..3760732 + 306 WP_042868396.1 type II toxin-antitoxin system toxin CcdB Toxin
Dpoa569_RS16925 3761162..3762889 + 1728 WP_042868394.1 site-specific DNA-methyltransferase -
Dpoa569_RS16930 3762880..3765513 + 2634 WP_042868392.1 DEAD/DEAH box helicase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3756268..3772559 16291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11735.57 Da        Isoelectric Point: 4.8500

>T135014 WP_042868396.1 NZ_CP042220:3760427-3760732 [Dickeya poaceiphila]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIESHHLSEKVNKTLFPLIRIDSEDYRLMTTELSSVPAEVIGEAI
ADLGDYADEIKDAINLMFWGI

Download         Length: 306 bp

>T135014 NZ_CP042220:3760427-3760732 [Dickeya poaceiphila]
ATGCAATTCATTGTTTATCAATACAAACGTGCCAGCCACTACAAAATGTTTGTTGATGTGCAGAGTGATATTGTCGAGAC
ACCTAAGCGGCGCATGGTCATACCTCTTATCGAATCGCATCACCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCTCTGA
TCCGCATCGACAGCGAAGACTATCGGCTGATGACGACGGAGCTATCGAGCGTTCCCGCTGAGGTCATTGGTGAGGCTATC
GCGGACCTTGGCGACTACGCCGATGAGATTAAGGATGCGATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8231.19 Da        Isoelectric Point: 7.2038

>AT135014 WP_038903500.1 NZ_CP042220:3760206-3760424 [Dickeya poaceiphila]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVTRFIAQHGSFADENRNW

Download         Length: 219 bp

>AT135014 NZ_CP042220:3760206-3760424 [Dickeya poaceiphila]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCCGCCGGAGTCAATATTTCTGGACT
GGTGAACGATGCAATTGGCAAAGAAGCCCGCCGCATTAAGGCAGAAGAGTGGAAAAAGGAAAACCGCGAAGGAATGGAAG
AAGTCACCCGATTTATCGCACAGCATGGCTCATTTGCAGATGAAAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B8IAT4

Antitoxin

Source ID Structure
AlphaFold DB A0A5B8IBF7

References