Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DhiAT/dnstrm_HI1420(antitoxin)
Location 2688829..2689582 Replicon chromosome
Accession NZ_CP042220
Organism Dickeya poaceiphila strain NCPPB 569

Toxin (Protein)


Gene name dhiT Uniprot ID A0A5B8IEM6
Locus tag Dpoa569_RS11940 Protein ID WP_042869741.1
Coordinates 2689325..2689582 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name dhiA Uniprot ID A0A5B8HP96
Locus tag Dpoa569_RS11935 Protein ID WP_042873857.1
Coordinates 2688829..2689317 (-) Length 163 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Dpoa569_RS11910 2684766..2686493 + 1728 WP_042869751.1 hydrogenase large subunit -
Dpoa569_RS11915 2686504..2687073 + 570 WP_042869749.1 hydrogenase 4 subunit H -
Dpoa569_RS11920 2687070..2687858 + 789 WP_042869747.1 NADH-quinone oxidoreductase subunit B family protein -
Dpoa569_RS11925 2687858..2688256 + 399 WP_042869745.1 formate hydrogenlyase maturation HycH family protein -
Dpoa569_RS11930 2688249..2688743 + 495 WP_042869743.1 hydrogenase maturation peptidase HycI -
Dpoa569_RS11935 2688829..2689317 - 489 WP_042873857.1 DUF2442 domain-containing protein Antitoxin
Dpoa569_RS11940 2689325..2689582 - 258 WP_042869741.1 DUF4160 domain-containing protein Toxin
Dpoa569_RS11945 2690050..2690592 + 543 WP_042869739.1 4Fe-4S dicluster domain-containing protein -
Dpoa569_RS11950 2690627..2692777 + 2151 WP_042869737.1 formate dehydrogenase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF4160

(9-74)

Antitoxin

Protein of unknown function DUF2442

(4-71)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 9958.52 Da        Isoelectric Point: 8.9518

>T135008 WP_042869741.1 NZ_CP042220:c2689582-2689325 [Dickeya poaceiphila]
MPEIDRLAGLIFHLYFYDDDRHHLPHVHVCYGNHELIINIGNGIEIEGYLPNNKRKLALSHIRAHRKQLLNMWERAVVGR
NPGKL

Download         Length: 258 bp

>T135008 NZ_CP042220:c2689582-2689325 [Dickeya poaceiphila]
ATGCCTGAGATTGACAGACTGGCGGGGTTAATCTTCCATTTGTACTTTTACGATGATGATCGGCACCACCTGCCGCATGT
GCATGTCTGCTACGGCAATCATGAGCTTATCATCAATATTGGTAACGGAATCGAGATCGAAGGATATCTGCCAAATAACA
AGCGCAAGCTGGCGCTTTCGCATATCAGGGCACATCGGAAACAACTGCTGAATATGTGGGAACGGGCGGTAGTGGGAAGA
AATCCGGGAAAACTGTGA

Antitoxin


Download         Length: 163 a.a.        Molecular weight: 17871.34 Da        Isoelectric Point: 6.9788

>AT135008 WP_042873857.1 NZ_CP042220:c2689317-2688829 [Dickeya poaceiphila]
MHKIVDVDVVGDFSLRLTYDDGLVCDVNLYHYASNPVFSNASLFIKFGLLPDGTLEWGPDVNISSSALRKKGEYVSHTLA
VRERNFADIISRAFYDAVMENRPEILQAALKIAVEKHGNSTVAKEAGAKSRTSLYKSLNKNTNVSLATVVSLAHTVLQLE
EK

Download         Length: 489 bp

>AT135008 NZ_CP042220:c2689317-2688829 [Dickeya poaceiphila]
ATGCATAAGATTGTAGACGTTGACGTTGTTGGCGATTTTTCGTTGCGCCTGACTTATGACGATGGCCTGGTGTGTGACGT
CAACCTTTATCACTACGCTTCAAACCCGGTGTTTTCGAATGCCAGCCTGTTTATCAAATTTGGGCTGTTGCCGGACGGCA
CGCTGGAATGGGGACCGGATGTCAACATTTCCTCGTCAGCATTACGGAAAAAAGGGGAGTATGTCAGCCACACACTTGCG
GTGCGGGAACGGAATTTTGCCGACATTATCTCCCGTGCCTTCTATGATGCTGTGATGGAAAATCGTCCTGAAATTCTGCA
AGCGGCGTTGAAAATTGCGGTGGAAAAACATGGCAACAGTACCGTAGCGAAAGAAGCGGGGGCGAAAAGCCGCACCAGCT
TATATAAGTCGCTGAATAAGAACACCAATGTCAGCCTGGCGACGGTAGTTAGCCTGGCGCACACCGTGCTGCAACTGGAA
GAAAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B8IEM6

Antitoxin

Source ID Structure
AlphaFold DB A0A5B8HP96

References