Detailed information of TA system TA134997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1450276..1450963
• Replicon: chromosome
• Accession: NZ_CP042220
• Organism: Dickeya poaceiphila strain NCPPB 569

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A5B8HJI1
• Locus tag: Dpoa569_RS06195
• Protein ID: WP_042871602.1
• Coordinates: 1450607..1450963 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: Dpoa569_RS06190
• Protein ID: WP_042871606.1
• Coordinates: 1450276..1450605 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Dpoa569_RS06175	1446729..1448756	-	2028	WP_042871614.1	hypothetical protein	-
Dpoa569_RS06180	1448753..1449217	-	465	WP_042871611.1	hypothetical protein	-
Dpoa569_RS06185	1449220..1450119	-	900	WP_042871609.1	hypothetical protein	-
Dpoa569_RS06190	1450276..1450605	-	330	WP_042871606.1	helix-turn-helix domain-containing protein	Antitoxin
Dpoa569_RS06195	1450607..1450963	-	357	WP_042871602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Dpoa569_RS06200	1451295..1451552	+	258	WP_042871601.1	hypothetical protein	-
Dpoa569_RS06205	1451549..1451833	+	285	WP_146411163.1	conjugal transfer protein TraD	-
Dpoa569_RS06210	1452046..1452300	-	255	WP_042871598.1	MafI family immunity protein	-
Dpoa569_RS06215	1452427..1452828	-	402	WP_026357825.1	H-NS histone family protein	-
Dpoa569_RS06220	1453011..1454615	+	1605	WP_050569476.1	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
Dpoa569_RS06225	1454707..1454901	-	195	WP_042871592.1	hypothetical protein	-
Dpoa569_RS06230	1455201..1455635	-	435	WP_128569753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1433215..1459100	25885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-87)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13450.54 Da
• Isoelectric Point: 9.2470

>T134997 WP_042871602.1 NZ_CP042220:c1450963-1450607 [Dickeya poaceiphila]
MLKNLYWIASARKDMKEMPEEVQDVFGYALHLAQCGKKHSDAKVMTGFSGAGVLEVVEDYETDTYRAVYTVKIEDAVYVL
HVFKKKSKKGIATPKPDMDKIRERLKQAEEHANSLRGK

Nucleotide

• Length: 357 bp

>T134997 NZ_CP042220:c1450963-1450607 [Dickeya poaceiphila]
ATGCTTAAAAATTTGTATTGGATTGCCAGCGCAAGGAAAGACATGAAGGAAATGCCTGAAGAGGTTCAGGATGTTTTCGG
CTATGCCCTGCATTTGGCTCAATGTGGGAAAAAGCACAGCGATGCCAAAGTGATGACCGGATTTAGCGGTGCTGGCGTTC
TTGAGGTCGTTGAGGATTACGAAACTGATACGTATCGGGCTGTTTATACAGTTAAAATTGAAGATGCCGTATATGTTTTG
CATGTGTTTAAGAAAAAATCTAAAAAAGGCATCGCAACACCAAAACCAGATATGGATAAGATCCGTGAAAGGTTGAAACA
GGCTGAAGAACACGCAAATAGTTTAAGAGGAAAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12187.15 Da
• Isoelectric Point: 6.9805

>AT134997 WP_042871606.1 NZ_CP042220:c1450605-1450276 [Dickeya poaceiphila]
MKNKIEVSSGNVYEDLDLPDSTTMFLKAQLASKITDIIKDNHYTQNQAAEILGIPQSKLSGLMRGQFRGVSELKMMECLL
RLGLDVEIVVREQSSEKKSSSLIPRVIFA

Nucleotide

• Length: 330 bp

>AT134997 NZ_CP042220:c1450605-1450276 [Dickeya poaceiphila]
ATGAAAAACAAAATCGAAGTTAGCAGTGGTAATGTCTATGAAGATCTGGATCTACCAGATTCAACAACTATGTTTTTAAA
GGCCCAGCTTGCATCCAAAATCACTGATATTATTAAAGATAACCACTATACACAAAATCAAGCAGCAGAAATTCTCGGTA
TCCCTCAATCCAAACTCTCCGGGCTGATGCGTGGACAGTTCCGAGGTGTTAGTGAGCTTAAAATGATGGAATGTCTACTC
AGACTTGGACTCGATGTTGAAATTGTTGTAAGAGAACAAAGTAGTGAAAAAAAATCATCCAGCCTAATCCCTAGGGTTAT
TTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A5B8HJI1