Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1108464..1109047 Replicon chromosome
Accession NZ_CP042220
Organism Dickeya poaceiphila strain NCPPB 569

Toxin (Protein)


Gene name mazF Uniprot ID A0A5B8I3P1
Locus tag Dpoa569_RS04950 Protein ID WP_042872014.1
Coordinates 1108464..1108796 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A5B8I221
Locus tag Dpoa569_RS04955 Protein ID WP_042872012.1
Coordinates 1108796..1109047 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Dpoa569_RS04905 1103615..1104073 - 459 WP_042872031.1 methylglyoxal synthase -
Dpoa569_RS04910 1104201..1104848 - 648 WP_042872029.1 DUF2057 family protein -
Dpoa569_RS04915 1105109..1105522 + 414 WP_042872027.1 CoA-binding protein -
Dpoa569_RS04920 1105598..1105921 - 324 WP_042872025.1 heat shock protein HspQ -
Dpoa569_RS04925 1106128..1106406 + 279 WP_042872022.1 acylphosphatase -
Dpoa569_RS04930 1106430..1106759 - 330 WP_042872019.1 sulfurtransferase TusE -
Dpoa569_RS04935 1106916..1107575 - 660 WP_042872017.1 FtsH protease modulator YccA -
Dpoa569_RS04945 1108084..1108380 + 297 Protein_944 hypothetical protein -
Dpoa569_RS04950 1108464..1108796 - 333 WP_042872014.1 endoribonuclease MazF Toxin
Dpoa569_RS04955 1108796..1109047 - 252 WP_042872012.1 PbsX family transcriptional regulator Antitoxin
Dpoa569_RS04960 1109292..1109504 + 213 WP_042872010.1 AlpA family transcriptional regulator -
Dpoa569_RS04965 1109861..1110235 + 375 Protein_948 IS630 family transposase -
Dpoa569_RS04970 1110296..1110571 + 276 Protein_949 GrpB family protein -
Dpoa569_RS04975 1110683..1111195 - 513 WP_042872008.1 GrpB family protein -
Dpoa569_RS04980 1111255..1111566 - 312 WP_042872006.1 hypothetical protein -
Dpoa569_RS04985 1111976..1112500 + 525 WP_042872005.1 isochorismatase family protein -
Dpoa569_RS04990 1112517..1112885 - 369 WP_042872003.1 DUF4186 domain-containing protein -
Dpoa569_RS04995 1113096..1113524 + 429 WP_042872001.1 universal stress protein -
Dpoa569_RS05000 1113770..1113994 - 225 WP_071604333.1 DUF2633 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1109720..1110235 515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

Antitoxin PemI-like

(3-82)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12120.89 Da        Isoelectric Point: 6.6949

>T134996 WP_042872014.1 NZ_CP042220:c1108796-1108464 [Dickeya poaceiphila]
MVSRFVPDSGDLIWLDFEPVVGHEQGGHRPAVVLSPFAYNNLTGMCLCVPCTTQRKGYSFEVELSGDRESVALADQVTCV
DWRNHKATKKGKVTTDELELIRAKAKALIG

Download         Length: 333 bp

>T134996 NZ_CP042220:c1108796-1108464 [Dickeya poaceiphila]
ATGGTTTCTCGTTTTGTTCCTGATTCCGGCGATTTAATTTGGTTAGATTTTGAACCGGTAGTGGGGCATGAACAGGGAGG
ACATCGCCCCGCTGTTGTTCTTAGCCCCTTTGCTTATAATAATTTGACCGGAATGTGCTTATGCGTCCCATGCACAACCC
AGCGCAAAGGGTATAGCTTCGAAGTCGAATTATCTGGAGACCGTGAAAGCGTGGCTCTCGCCGATCAGGTCACTTGTGTA
GACTGGCGTAACCATAAGGCAACAAAGAAAGGCAAAGTGACTACCGATGAGTTAGAACTCATCAGAGCAAAAGCTAAGGC
TTTGATTGGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9458.97 Da        Isoelectric Point: 4.8047

>AT134996 WP_042872012.1 NZ_CP042220:c1109047-1108796 [Dickeya poaceiphila]
MIDVPVKKWGNSPAIRLPSSVIQAFDMTFNDSFDMEIRETEIALIPKKKPKYQFTLDELLEGMTPEHVHEKVDFGAPVGK
ELL

Download         Length: 252 bp

>AT134996 NZ_CP042220:c1109047-1108796 [Dickeya poaceiphila]
ATGATCGATGTTCCAGTTAAAAAATGGGGAAACTCACCTGCAATACGTCTTCCCTCATCTGTTATACAAGCGTTTGATAT
GACATTTAACGATAGCTTTGACATGGAGATCAGGGAAACAGAGATTGCTCTGATCCCCAAGAAAAAACCCAAGTATCAGT
TTACGCTGGATGAGTTGCTCGAAGGGATGACGCCTGAACATGTCCATGAGAAAGTTGATTTTGGCGCACCTGTCGGCAAG
GAGCTGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B8I3P1

Antitoxin

Source ID Structure
AlphaFold DB A0A5B8I221

References