Detailed information of TA system TA134990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 13300..13871
• Replicon: plasmid pL8-A
• Accession: NZ_CP042217
• Organism: Enterococcus faecalis strain L8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FOA09_RS13510
• Protein ID: WP_010717284.1
• Coordinates: 13530..13871 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: FOA09_RS13505
• Protein ID: WP_002362431.1
• Coordinates: 13300..13530 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA09_RS13460	8860..9156	-	297	WP_002367795.1	hypothetical protein	-
FOA09_RS13465	9413..9499	-	87	WP_012881081.1	type I toxin-antitoxin system Fst family toxin	-
FOA09_RS13470	9754..10272	-	519	WP_002367793.1	hypothetical protein	-
FOA09_RS13475	10688..11029	-	342	WP_002367792.1	bacteriocin immunity protein	-
FOA09_RS13480	11030..11245	-	216	WP_002367791.1	leucocin A/sakacin P family class II bacteriocin	-
FOA09_RS13485	11415..11606	-	192	WP_002367788.1	hypothetical protein	-
FOA09_RS13490	11618..11812	-	195	WP_002367787.1	hypothetical protein	-
FOA09_RS13495	12172..12486	-	315	WP_002367785.1	hypothetical protein	-
FOA09_RS13500	12476..13096	-	621	WP_002367784.1	recombinase family protein	-
FOA09_RS13505	13300..13530	+	231	WP_002362431.1	hypothetical protein	Antitoxin
FOA09_RS13510	13530..13871	+	342	WP_010717284.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOA09_RS13515	13983..14921	+	939	WP_002362433.1	hypothetical protein	-
FOA09_RS13520	15186..15788	+	603	WP_002362434.1	Fic family protein	-
FOA09_RS13525	15927..17065	+	1139	WP_094467193.1	IS3 family transposase	-
FOA09_RS13530	17190..18473	+	1284	WP_002362438.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(L)	-	1..85951	85951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13212.44 Da
• Isoelectric Point: 8.8196

>T134990 WP_010717284.1 NZ_CP042217:13530-13871 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEYLPLKDMAKIDQIIAYIF

Nucleotide

• Length: 342 bp

>T134990 NZ_CP042217:13530-13871 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAATATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGCATACATTTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT134990 WP_002362431.1 NZ_CP042217:13300-13530 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT134990 NZ_CP042217:13300-13530 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTAATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Antitoxin

• AlphaFold DB: R3KHK9