Detailed information of TA system TA134982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2716006..2716577
• Replicon: chromosome
• Accession: NZ_CP042216
• Organism: Enterococcus faecalis strain L8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FOA09_RS13040
• Protein ID: WP_002354774.1
• Coordinates: 2716006..2716347 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: FOA09_RS13045
• Protein ID: WP_002354773.1
• Coordinates: 2716347..2716577 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA09_RS13035	2712021..2715635	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
FOA09_RS13040	2716006..2716347	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOA09_RS13045	2716347..2716577	-	231	WP_002354773.1	hypothetical protein	Antitoxin
FOA09_RS13050	2716991..2717206	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
FOA09_RS13055	2717345..2718337	+	993	WP_016632734.1	biotin--[acetyl-CoA-carboxylase] ligase	-
FOA09_RS13060	2718404..2719036	-	633	WP_002358972.1	RloB family protein	-
FOA09_RS13065	2719045..2720340	-	1296	WP_002410675.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T134982 WP_002354774.1 NZ_CP042216:c2716347-2716006 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T134982 NZ_CP042216:c2716347-2716006 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT134982 WP_002354773.1 NZ_CP042216:c2716577-2716347 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT134982 NZ_CP042216:c2716577-2716347 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812