Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-DUF971
Location 8332..8933 Replicon plasmid unnamed
Accession NZ_CP042181
Organism Pseudomonas sp. KBS0802

Toxin (Protein)


Gene name PumA Uniprot ID Q8VMN7
Locus tag FFH79_RS29250 Protein ID WP_011005862.1
Coordinates 8631..8933 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID Q8VMN8
Locus tag FFH79_RS29245 Protein ID WP_011005861.1
Coordinates 8332..8634 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFH79_RS29220 3597..4883 - 1287 WP_090315698.1 Y-family DNA polymerase -
FFH79_RS29225 4873..5304 - 432 WP_011005854.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
FFH79_RS29230 5442..6392 + 951 WP_011005855.1 non-homologous end-joining DNA ligase -
FFH79_RS29235 6423..6698 + 276 WP_011005856.1 hypothetical protein -
FFH79_RS29240 8067..8291 - 225 WP_011005860.1 hypothetical protein -
FFH79_RS29245 8332..8634 - 303 WP_011005861.1 putative addiction module antidote protein Antitoxin
FFH79_RS29250 8631..8933 - 303 WP_011005862.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FFH79_RS29255 9135..9914 + 780 WP_011005863.1 DUF4942 domain-containing protein -
FFH79_RS29260 10068..10436 + 369 WP_011005864.1 hypothetical protein -
FFH79_RS29265 10469..10786 + 318 WP_062378618.1 hypothetical protein -
FFH79_RS29270 10842..11060 + 219 WP_011005866.1 hypothetical protein -
FFH79_RS29275 11199..12218 + 1020 WP_062378625.1 hypothetical protein -
FFH79_RS29280 12361..12603 + 243 WP_011005868.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..116559 116559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(7-95)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11291.87 Da        Isoelectric Point: 10.3903

>T134974 WP_011005862.1 NZ_CP042181:c8933-8631 [Pseudomonas sp. KBS0802]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVAHGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKTLASQWSDDE

Download         Length: 303 bp

>T134974 NZ_CP042181:c8933-8631 [Pseudomonas sp. KBS0802]
ATGAAGACAATAAAACAAACCGCCACTTACAGGAACTGGGAACGCAAGCTGCGGGATAAGCAGGCCAAAGCCATCATCGC
TGCGCGAGTCTTCCGCGTTGCACATGGTTTGCTTGGAGACGTTCAACCCGTGGGCCAAGGGATCAGCGAACTGCGTATCC
ATCACGGCCCCGGCTACCGCGTGTACTTCCAGCAGCGGGGTAACCAACTGGTTCTGTTGCTGTGTGGTGGGGACAAGAGC
AGCCAAGCGCGGGATATCGAAACCGCAAAAACCTTGGCCAGCCAGTGGAGTGACGATGAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 10967.43 Da        Isoelectric Point: 5.1084

>AT134974 WP_011005861.1 NZ_CP042181:c8634-8332 [Pseudomonas sp. KBS0802]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHIEPAHQ

Download         Length: 303 bp

>AT134974 NZ_CP042181:c8634-8332 [Pseudomonas sp. KBS0802]
ATGAAAGAGCAATTTTACGACTATGACCCCGCTGATGCGCTGACCGATGCGGAGTCCATCGCGGTTTTCATGGAGGACGC
ATTCGAAACGGGCGATGCGGCGTATATCGCTAAATGCCTGGGTGTCGTGGCTCGTGCCAAAGGCATGACCGCCCTTGCCA
ATGAATCCGGCCTTTCCCGCGAACACCTTTACCGGTCGTTCTCGGACAAGGGCAATCCAACCTTGAAAACCTTGCTGGCC
GTGATGCGCGCCGTCGGCTTGGATCTCACGACCCGAAAGCACATCGAGCCGGCGCACCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4KM11

Antitoxin

Source ID Structure
AlphaFold DB Q8VMN8

References