Detailed information of TA system TA134965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 325237..326105
• Replicon: chromosome
• Accession: NZ_CP042180
• Organism: Pseudomonas sp. KBS0802

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FFH79_RS01445
• Protein ID: WP_061405554.1
• Coordinates: 325237..325590 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q88R60
• Locus tag: FFH79_RS01450
• Protein ID: WP_004575922.1
• Coordinates: 325632..326105 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFH79_RS01425	320257..321624	-	1368	WP_010951639.1	HAMP domain-containing protein	-
FFH79_RS01430	321626..322345	-	720	WP_010951640.1	response regulator	-
FFH79_RS01435	322486..324783	+	2298	WP_010951641.1	TonB-dependent siderophore receptor	-
FFH79_RS01440	324849..325055	-	207	WP_014860378.1	DUF3079 domain-containing protein	-
FFH79_RS01445	325237..325590	+	354	WP_061405554.1	type II toxin-antitoxin system HigB family toxin	Toxin
FFH79_RS01450	325632..326105	+	474	WP_004575922.1	transcriptional regulator	Antitoxin
FFH79_RS01455	326110..326297	-	188	Protein_272	integrase	-
FFH79_RS01460	326525..326740	+	216	WP_010951644.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FFH79_RS01465	326737..327060	-	324	WP_010951645.1	helix-turn-helix domain-containing protein	-
FFH79_RS01475	327522..327770	-	249	WP_010951646.1	hypothetical protein	-
FFH79_RS01480	328291..328566	+	276	WP_049588069.1	DUF3077 domain-containing protein	-
FFH79_RS01485	328921..330126	-	1206	WP_010951648.1	methyltransferase	-
FFH79_RS01490	330255..330944	-	690	WP_010951649.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13672.88 Da
• Isoelectric Point: 10.6182

>T134965 WP_061405554.1 NZ_CP042180:325237-325590 [Pseudomonas sp. KBS0802]
MRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 354 bp

>T134965 NZ_CP042180:325237-325590 [Pseudomonas sp. KBS0802]
ATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGATTGAAGTCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCT
GACGACCTTCGACAGGGCGACGCTGCGGTTCGAATCTTTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGA
ATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAG
AAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGA
GTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17229.67 Da
• Isoelectric Point: 4.4982

>AT134965 WP_004575922.1 NZ_CP042180:325632-326105 [Pseudomonas sp. KBS0802]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT134965 NZ_CP042180:325632-326105 [Pseudomonas sp. KBS0802]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGATAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CBJ4