Detailed information of TA system TA134931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 460007..460601
• Replicon: chromosome
• Accession: NZ_CP042170
• Organism: Flavobacterium sp. KBS0721

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S9A5K1
• Locus tag: B0M43_RS02220
• Protein ID: WP_065450827.1
• Coordinates: 460359..460601 (-)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: B0M43_RS02215
• Protein ID: WP_078006182.1
• Coordinates: 460007..460357 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0M43_RS02195	455648..456133	+	486	WP_078006016.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
B0M43_RS02200	456270..456938	-	669	WP_078006017.1	hypothetical protein	-
B0M43_RS02205	457162..459189	+	2028	WP_078006018.1	M3 family metallopeptidase	-
B0M43_RS02210	459325..459984	+	660	WP_078006019.1	YdcF family protein	-
B0M43_RS02215	460007..460357	-	351	WP_078006182.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B0M43_RS02220	460359..460601	-	243	WP_065450827.1	type II toxin-antitoxin system HicA family toxin	Toxin
B0M43_RS02225	460795..462057	-	1263	WP_078006020.1	sodium:proton antiporter	-
B0M43_RS02230	462338..463816	-	1479	WP_078006021.1	hypothetical protein	-
B0M43_RS02235	463965..464378	-	414	WP_078006022.1	DUF1842 domain-containing protein	-
B0M43_RS02240	464790..465293	+	504	WP_078006023.1	ArsR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-79)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 9401.00 Da
• Isoelectric Point: 10.2439

>T134931 WP_065450827.1 NZ_CP042170:c460601-460359 [Flavobacterium sp. KBS0721]
MSKIDKLIEKLKSKPKDFSWDEMLKVLNHFGYKQISQGKTGGSRRKFVNENKQIISLHEPHPQKVLKGYQLDIIIEHLEL

Nucleotide

• Length: 243 bp

>T134931 NZ_CP042170:c460601-460359 [Flavobacterium sp. KBS0721]
ATGAGTAAAATTGATAAACTTATTGAAAAGTTAAAATCAAAACCAAAAGATTTTTCTTGGGATGAAATGCTGAAAGTTCT
AAATCATTTCGGTTATAAACAAATCTCACAAGGAAAAACAGGAGGCTCAAGAAGAAAATTTGTGAATGAAAATAAACAGA
TTATAAGTCTGCACGAACCGCATCCACAAAAAGTTTTAAAAGGATACCAACTTGATATTATTATTGAACATTTGGAATTA
TAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13303.14 Da
• Isoelectric Point: 4.9691

>AT134931 WP_078006182.1 NZ_CP042170:c460357-460007 [Flavobacterium sp. KBS0721]
MKNYLEYNGYIGTLEFSADDKIFFGKIQGINDLITFEGYSVNELEKSFEEAVDDYLETCKLLNKAPDKSYKGSFNVRVSQ
ELHRKIALLATKKGLNLNEVVKEALSYIIKHEEVLN

Nucleotide

• Length: 351 bp

>AT134931 NZ_CP042170:c460357-460007 [Flavobacterium sp. KBS0721]
ATGAAAAACTATTTAGAATATAACGGATACATTGGTACATTAGAATTTTCTGCAGATGATAAAATATTCTTTGGAAAAAT
TCAAGGTATAAACGATTTAATAACCTTCGAAGGTTATTCTGTTAACGAATTAGAAAAATCTTTTGAAGAAGCTGTTGACG
ACTATTTAGAAACATGTAAATTATTGAATAAAGCTCCAGATAAAAGTTATAAAGGATCTTTTAATGTAAGAGTTTCTCAG
GAACTACACCGGAAAATAGCTCTCTTAGCCACTAAAAAAGGATTGAATTTAAATGAGGTCGTAAAGGAAGCACTATCTTA
CATAATTAAACACGAAGAGGTATTGAACTAA

Structures

Toxin

• AlphaFold DB: A0A1S9A5K1