Detailed information of TA system TA134928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 66811..67460
• Replicon: plasmid unnamed1
• Accession: NZ_CP042167
• Organism: Burkholderia contaminans strain ZCC

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPQ37_RS40590
• Protein ID: WP_027813527.1
• Coordinates: 66811..67221 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6P2T8K5
• Locus tag: FPQ37_RS40595
• Protein ID: WP_027813526.1
• Coordinates: 67218..67460 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPQ37_RS40555	62358..63206	-	849	WP_027813534.1	heteromeric transposase endonuclease subunit TnsA	-
FPQ37_RS40560	63366..63596	-	231	WP_027813533.1	hypothetical protein	-
FPQ37_RS40565	63607..64692	-	1086	WP_027813532.1	hypothetical protein	-
FPQ37_RS40570	64737..65042	-	306	WP_027813531.1	hypothetical protein	-
FPQ37_RS40575	65063..65715	-	653	Protein_66	helix-turn-helix domain-containing protein	-
FPQ37_RS40580	65903..66103	-	201	WP_034176085.1	hypothetical protein	-
FPQ37_RS40585	66152..66499	-	348	WP_027813528.1	hypothetical protein	-
FPQ37_RS40590	66811..67221	-	411	WP_027813527.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPQ37_RS40595	67218..67460	-	243	WP_027813526.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FPQ37_RS40600	67555..68604	-	1050	WP_027813525.1	non-homologous end-joining DNA ligase	-
FPQ37_RS40605	69007..69678	+	672	WP_027813524.1	AAA family ATPase	-
FPQ37_RS40610	69678..70643	+	966	WP_027813523.1	ParB/RepB/Spo0J family partition protein	-
FPQ37_RS40615	71386..72243	+	858	WP_027813522.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..239952	239952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14869.13 Da
• Isoelectric Point: 4.8604

>T134928 WP_027813527.1 NZ_CP042167:c67221-66811 [Burkholderia contaminans]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIDANDMLIAAHALAVDAVLVTDNTAEFTRIAGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T134928 NZ_CP042167:c67221-66811 [Burkholderia contaminans]
ATGACGCTGTACCTGCTCGATACCAACATTTTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCACCCGGATTGG
AGAAACGCCGCCCGAACAAGTGTGCACCTCGATCATCGTGGCCGCGGAATTGCGGTTCGGCGTCTGGAAGCGTGGCTCGT
CAACGCTCGCGCAGCGCGTCGAGCAGCTGCTCGCAAGCCTGACCGTGTTGCCGCTGCAACCGGACGCGGACCGCTGCTAC
GGGCGGTTGCGGGCCGAGCTCGAGAAGCAAGGCCAGTTGATCGACGCCAACGACATGCTGATCGCCGCGCATGCGCTGGC
CGTCGACGCCGTGCTGGTCACGGACAACACGGCTGAATTCACCCGTATCGCGGGGCTGCCGGTCGAAAACTGGCTGCGGC
CGGCGACGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9375.64 Da
• Isoelectric Point: 4.6856

>AT134928 WP_027813526.1 NZ_CP042167:c67460-67218 [Burkholderia contaminans]
MPNHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDEIFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT134928 NZ_CP042167:c67460-67218 [Burkholderia contaminans]
ATGCCAAACCATGACACCCGCCACGCGCGCCTGTTCCGCAACGGGCGGAGCCAGGCCGTGCGGATCCCGCGCGAATTCGA
ACTGCCGGTGTCGGAGGTCACCATCCACCGGGAAGGTCGCCGCTTGATCATTGAGCCCGTAGAGGCCGCGCCCTTGCGCG
AGCTGTTCGCCCAATGGACCCCGCTGGACGAGATCTTCCCGGACATCGAGGACCTTCCGCCTGAACCGGTCGATATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A6P2T8K5