Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4545345..4545998 Replicon chromosome
Accession NZ_CP042161
Organism Brevibacillus brevis strain HK544

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag FPS98_RS21755 Protein ID WP_005830205.1
Coordinates 4545345..4545695 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A291BDF6
Locus tag FPS98_RS21760 Protein ID WP_016740787.1
Coordinates 4545699..4545998 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPS98_RS21730 4542099..4542887 - 789 WP_048036230.1 RNA polymerase sigma factor SigB -
FPS98_RS21735 4542856..4543350 - 495 WP_144617968.1 anti-sigma B factor RsbW -
FPS98_RS21740 4543350..4543676 - 327 WP_069852126.1 anti-sigma factor antagonist -
FPS98_RS21745 4543701..4544702 - 1002 WP_144617969.1 PP2C family protein-serine/threonine phosphatase -
FPS98_RS21750 4544708..4545136 - 429 WP_144617970.1 anti-sigma regulatory factor -
FPS98_RS21755 4545345..4545695 - 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FPS98_RS21760 4545699..4545998 - 300 WP_016740787.1 CopG family ribbon-helix-helix protein Antitoxin
FPS98_RS21765 4546228..4547430 - 1203 WP_144617971.1 alanine racemase -
FPS98_RS21770 4547433..4547873 - 441 WP_144617972.1 hypothetical protein -
FPS98_RS21775 4548058..4549170 - 1113 WP_144617973.1 hypothetical protein -
FPS98_RS21785 4549781..4550818 - 1038 WP_144617974.1 outer membrane lipoprotein-sorting protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T134919 WP_005830205.1 NZ_CP042161:c4545695-4545345 [Brevibacillus brevis]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

>T134919 NZ_CP042161:c4545695-4545345 [Brevibacillus brevis]
GTGATTGTCAAACGTGGCGATGTGTTTTTCGCGGACCTGTCCCCGGTCGTTGGCTCTGAGCAAGGAGGCGTACGGCCTGT
TTTAGTCATTCAAAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCAGCCATTACGGCGCAGATTCAAAAGG
CGAAGCTGCCTACGCATGTGGAAATTGACGCAAAAACTTATGGATTTGATCGTGACTCCGTCATTCTGTTGGAACAAATA
CGAACCATTGACAAACAACGTCTAACTGATAAAATAACGCATCTCGATGACGAGATGATGGATCGGGTTAATGAATCCTT
GCAAATCAGTTTAGGACTCATTGATTTTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11308.20 Da        Isoelectric Point: 10.4095

>AT134919 WP_016740787.1 NZ_CP042161:c4545998-4545699 [Brevibacillus brevis]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLNMASE
AFGAEQEADHTLVRLVSGV

Download         Length: 300 bp

>AT134919 NZ_CP042161:c4545998-4545699 [Brevibacillus brevis]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGCTTGCCACAACACTTGCTTCAGGA
AGTGGATGGCGTTATTCAAAAGGAAAAATCCAATCGCAGCGAGTTTATTCGTCAAGCGATGCATTTGTATTTGAAAGAGC
GAAAAAAACGCACAATCCGTGAGACCATGCAAAAGGGGTACATGGAGATGGCGAAGATCAATCTGAATATGGCATCGGAG
GCTTTTGGCGCTGAACAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A291BDF6

References