Detailed information of TA system TA134906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 133338..133867
• Replicon: chromosome
• Accession: NZ_CP042157
• Organism: Staphylococcus aureus strain B3-17D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FP481_RS00775
• Protein ID: WP_000621175.1
• Coordinates: 133338..133700 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FP481_RS00780
• Protein ID: WP_000948331.1
• Coordinates: 133697..133867 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FP481_RS00750	128874..130193	+	1320	WP_001557163.1	ISL3-like element IS1181 family transposase	-
FP481_RS00755	130316..131086	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
FP481_RS00760	131061..131540	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FP481_RS00765	131542..131868	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FP481_RS00770	131987..132988	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FP481_RS00775	133338..133700	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FP481_RS00780	133697..133867	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FP481_RS00785	133952..135100	-	1149	WP_001281154.1	alanine racemase	-
FP481_RS00790	135166..135525	-	360	WP_000581197.1	holo-ACP synthase	-
FP481_RS00795	135529..136020	-	492	WP_001205912.1	PH domain-containing protein	-
FP481_RS00800	136007..137590	-	1584	WP_001294626.1	PH domain-containing protein	-
FP481_RS00805	137583..138062	-	480	WP_001287087.1	hypothetical protein	-
FP481_RS00810	138270..138830	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	128874..130193	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T134906 WP_000621175.1 NZ_CP042157:c133700-133338 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T134906 NZ_CP042157:c133700-133338 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT134906 WP_000948331.1 NZ_CP042157:c133867-133697 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT134906 NZ_CP042157:c133867-133697 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1