Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF-doc
Location 7767..8371 Replicon plasmid pSALNC14
Accession NZ_CP042154
Organism Staphylococcus aureus strain B4-59C

Toxin (Protein)


Gene name pemK Uniprot ID L7PFJ6
Locus tag FP482_RS14115 Protein ID WP_001022435.1
Coordinates 8033..8371 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID L7PH55
Locus tag FP482_RS14110 Protein ID WP_001129937.1
Coordinates 7767..8036 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FP482_RS14095 4871..5239 + 369 WP_041203784.1 hypothetical protein -
FP482_RS14100 5543..6274 - 732 WP_000753929.1 CPBP family intramembrane metalloprotease -
FP482_RS14105 6387..7196 - 810 WP_001106667.1 alpha/beta hydrolase -
FP482_RS14110 7767..8036 + 270 WP_001129937.1 hypothetical protein Antitoxin
FP482_RS14115 8033..8371 + 339 WP_001022435.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FP482_RS14120 8546..9229 + 684 WP_000277644.1 CPBP family intramembrane metalloprotease -
FP482_RS14125 9808..10482 + 675 WP_001106066.1 IS6 family transposase -
FP482_RS14130 10634..11044 - 411 WP_114306678.1 hypothetical protein -
FP482_RS14135 11054..11287 - 234 WP_001002995.1 hypothetical protein -
FP482_RS14140 11319..12602 - 1284 WP_000083517.1 replication initiator protein A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..14156 14156
- flank IS/Tn - - 9808..10482 674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13001.20 Da        Isoelectric Point: 10.1988

>T134902 WP_001022435.1 NZ_CP042154:8033-8371 [Staphylococcus aureus]
MNIKQFDILYIDLNPTRGREKHNVRPCLVINNQMSIDGTNFVWVLPITTRGLRYPTDIQLKTKKGLVSGVIDTVQIRALD
LKARQYNYKDELQDNLKNDILKAIKTYLKPTL

Download         Length: 339 bp

>T134902 NZ_CP042154:8033-8371 [Staphylococcus aureus]
ATGAACATTAAACAATTTGACATACTCTACATTGATTTAAATCCAACTCGTGGACGTGAAAAGCATAACGTAAGGCCCTG
CTTAGTGATAAATAATCAGATGAGTATTGATGGCACTAATTTTGTTTGGGTTTTACCCATAACAACTCGGGGTTTACGTT
ATCCAACAGATATCCAACTTAAAACAAAAAAAGGATTAGTATCTGGTGTGATAGACACTGTTCAAATACGCGCTTTAGAC
CTAAAAGCACGTCAATACAACTATAAAGACGAACTACAGGACAATTTAAAAAATGATATATTAAAAGCGATAAAAACTTA
TCTTAAGCCAACGTTATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10185.65 Da        Isoelectric Point: 10.1641

>AT134902 WP_001129937.1 NZ_CP042154:7767-8036 [Staphylococcus aureus]
MAKSLKDLNKTSERVFKNGNSKAISLSKKTIRLANFDIGDTVEVQKMNGGLFITKKKESIEDRIKNFFQSGGKYTELEVD
WEERVGREI

Download         Length: 270 bp

>AT134902 NZ_CP042154:7767-8036 [Staphylococcus aureus]
ATGGCTAAATCATTAAAAGACTTAAACAAGACTTCGGAACGAGTTTTTAAAAATGGAAACAGTAAAGCAATCAGTTTAAG
TAAAAAAACTATCAGGTTAGCTAATTTTGATATAGGAGATACAGTTGAAGTTCAAAAAATGAATGGTGGTTTGTTCATTA
CTAAAAAAAAGGAGTCTATTGAAGATAGAATTAAAAATTTCTTTCAAAGTGGTGGAAAGTATACAGAATTAGAAGTAGAT
TGGGAAGAAAGAGTTGGCCGAGAAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7EWJ
PDB 7EWI

Antitoxin

Source ID Structure
PDB 7EWJ
AlphaFold DB L7PH55

References