Detailed information of TA system TA134894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2174439..2174968
• Replicon: chromosome
• Accession: NZ_CP042153
• Organism: Staphylococcus aureus strain B4-59C

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FP482_RS10880
• Protein ID: WP_000621175.1
• Coordinates: 2174439..2174801 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FP482_RS10885
• Protein ID: WP_000948331.1
• Coordinates: 2174798..2174968 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FP482_RS10855	2169975..2171294	+	1320	WP_145411605.1	ISL3-like element IS1181 family transposase	-
FP482_RS10860	2171417..2172187	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
FP482_RS10865	2172162..2172641	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FP482_RS10870	2172643..2172969	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FP482_RS10875	2173088..2174089	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FP482_RS10880	2174439..2174801	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FP482_RS10885	2174798..2174968	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FP482_RS10890	2175053..2176201	-	1149	WP_001281154.1	alanine racemase	-
FP482_RS10895	2176267..2176626	-	360	WP_000581197.1	holo-ACP synthase	-
FP482_RS10900	2176630..2177121	-	492	WP_001205912.1	PH domain-containing protein	-
FP482_RS10905	2177108..2178691	-	1584	WP_001294626.1	PH domain-containing protein	-
FP482_RS10910	2178684..2179163	-	480	WP_001287087.1	hypothetical protein	-
FP482_RS10915	2179371..2179931	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2169975..2171294	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T134894 WP_000621175.1 NZ_CP042153:c2174801-2174439 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T134894 NZ_CP042153:c2174801-2174439 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT134894 WP_000948331.1 NZ_CP042153:c2174968-2174798 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT134894 NZ_CP042153:c2174968-2174798 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1