Detailed information of TA system TA134863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 239099..239628
• Replicon: chromosome
• Accession: NZ_CP042046
• Organism: Staphylococcus aureus strain B2-7A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FP477_RS01445
• Protein ID: WP_000621175.1
• Coordinates: 239099..239461 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FP477_RS01450
• Protein ID: WP_000948331.1
• Coordinates: 239458..239628 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FP477_RS01425	236078..236848	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
FP477_RS01430	236823..237302	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FP477_RS01435	237304..237630	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FP477_RS01440	237749..238750	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FP477_RS01445	239099..239461	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FP477_RS01450	239458..239628	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FP477_RS01455	239713..240861	-	1149	WP_001281151.1	alanine racemase	-
FP477_RS01460	240927..241286	-	360	WP_000581200.1	holo-ACP synthase	-
FP477_RS01465	241290..241781	-	492	WP_001205910.1	PH domain-containing protein	-
FP477_RS01470	241768..243351	-	1584	WP_001294626.1	PH domain-containing protein	-
FP477_RS01475	243344..243823	-	480	WP_001287090.1	hypothetical protein	-
FP477_RS01480	244032..244592	-	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T134863 WP_000621175.1 NZ_CP042046:c239461-239099 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T134863 NZ_CP042046:c239461-239099 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT134863 WP_000948331.1 NZ_CP042046:c239628-239458 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT134863 NZ_CP042046:c239628-239458 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1