Detailed information of TA system TA134853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2368094..2368623
• Replicon: chromosome
• Accession: NZ_CP042043
• Organism: Staphylococcus aureus strain B2-15A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FP478_RS11930
• Protein ID: WP_000621175.1
• Coordinates: 2368094..2368456 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FP478_RS11935
• Protein ID: WP_000948331.1
• Coordinates: 2368453..2368623 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FP478_RS11905	2363630..2364949	+	1320	WP_001557163.1	ISL3-like element IS1181 family transposase	-
FP478_RS11910	2365072..2365842	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
FP478_RS11915	2365817..2366296	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FP478_RS11920	2366298..2366624	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FP478_RS11925	2366743..2367744	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FP478_RS11930	2368094..2368456	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FP478_RS11935	2368453..2368623	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FP478_RS11940	2368708..2369856	-	1149	WP_001281154.1	alanine racemase	-
FP478_RS11945	2369922..2370281	-	360	WP_000581197.1	holo-ACP synthase	-
FP478_RS11950	2370285..2370776	-	492	WP_001205912.1	PH domain-containing protein	-
FP478_RS11955	2370763..2372346	-	1584	WP_001294626.1	PH domain-containing protein	-
FP478_RS11960	2372339..2372818	-	480	WP_001287087.1	hypothetical protein	-
FP478_RS11965	2373026..2373586	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2363630..2364949	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T134853 WP_000621175.1 NZ_CP042043:c2368456-2368094 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T134853 NZ_CP042043:c2368456-2368094 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT134853 WP_000948331.1 NZ_CP042043:c2368623-2368453 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT134853 NZ_CP042043:c2368623-2368453 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1