Detailed information of TA system TA134835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 911075..911604
• Replicon: chromosome
• Accession: NZ_CP042003
• Organism: Staphylococcus aureus strain B3-14B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FP480_RS04450
• Protein ID: WP_000621175.1
• Coordinates: 911242..911604 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FP480_RS04445
• Protein ID: WP_000948331.1
• Coordinates: 911075..911245 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FP480_RS04415	906111..906671	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
FP480_RS04420	906880..907359	+	480	WP_001287081.1	hypothetical protein	-
FP480_RS04425	907352..908929	+	1578	WP_001294650.1	PH domain-containing protein	-
FP480_RS04430	908922..909413	+	492	WP_001286980.1	PH domain-containing protein	-
FP480_RS04435	909417..909776	+	360	WP_000581197.1	holo-ACP synthase	-
FP480_RS04440	909842..910990	+	1149	WP_001281139.1	alanine racemase	-
FP480_RS04445	911075..911245	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FP480_RS04450	911242..911604	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FP480_RS04455	911953..912954	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FP480_RS04460	913073..913399	+	327	WP_001052491.1	anti-sigma factor antagonist	-
FP480_RS04465	913401..913880	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
FP480_RS04470	913855..914625	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T134835 WP_000621175.1 NZ_CP042003:911242-911604 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T134835 NZ_CP042003:911242-911604 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT134835 WP_000948331.1 NZ_CP042003:911075-911245 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT134835 NZ_CP042003:911075-911245 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1