Detailed information of TA system TA134834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 891353..891867
• Replicon: chromosome
• Accession: NZ_CP042003
• Organism: Staphylococcus aureus strain B3-14B

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: FP480_RS04360
• Protein ID: WP_000581792.1
• Coordinates: 891658..891867 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: FP480_RS04355
• Protein ID: WP_000138932.1
• Coordinates: 891353..891646 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FP480_RS04325	886582..886884	-	303	WP_000572393.1	hypothetical protein	-
FP480_RS04330	887041..887325	-	285	WP_000807442.1	hypothetical protein	-
FP480_RS04335	887377..888431	+	1055	Protein_834	ISL3 family transposase	-
FP480_RS04340	888428..888748	-	321	WP_001825748.1	hypothetical protein	-
FP480_RS04345	889028..889675	-	648	WP_001187614.1	HD domain-containing protein	-
FP480_RS04350	889703..891187	-	1485	WP_000571550.1	cardiolipin synthase	-
FP480_RS04355	891353..891646	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
FP480_RS04360	891658..891867	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
FP480_RS04365	891995..892132	+	138	WP_000828354.1	hypothetical protein	-
FP480_RS04370	892396..893598	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
FP480_RS04375	893916..894986	+	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
FP480_RS04380	895001..896356	+	1356	WP_000611457.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	834840..898511	63671
	-	flank	IS/Tn	-	-	887520..888431	911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T134834 WP_000581792.1 NZ_CP042003:891658-891867 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T134834 NZ_CP042003:891658-891867 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT134834 WP_000138932.1 NZ_CP042003:891353-891646 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT134834 NZ_CP042003:891353-891646 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13