Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4162294..4162870 Replicon chromosome
Accession NZ_CP041996
Organism Escherichia coli strain AR Bank #0349

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag FN958_RS20195 Protein ID WP_001131965.1
Coordinates 4162583..4162870 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag FN958_RS20190 Protein ID WP_000063158.1
Coordinates 4162294..4162596 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FN958_RS20170 4157402..4158622 + 1221 WP_000873756.1 amino acid deaminase -
FN958_RS20175 4158890..4161040 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
FN958_RS20180 4161090..4161293 + 204 WP_000467859.1 YbdD/YjiX family protein -
FN958_RS20185 4161304..4162260 + 957 WP_001361361.1 GTPase -
FN958_RS20190 4162294..4162596 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
FN958_RS20195 4162583..4162870 - 288 WP_001131965.1 BrnT family toxin Toxin
FN958_RS20200 4163067..4164233 + 1167 WP_000800823.1 restriction endonuclease -
FN958_RS20205 4164278..4164451 + 174 Protein_3924 SAM-dependent DNA methyltransferase -
FN958_RS20210 4164445..4165113 + 669 Protein_3925 DUF3387 domain-containing protein -
FN958_RS20215 4165328..4165666 + 339 WP_000132633.1 endoribonuclease SymE -
FN958_RS20220 4165708..4165872 - 165 WP_000394263.1 DUF1127 domain-containing protein -
FN958_RS20225 4166049..4167461 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T134801 WP_001131965.1 NZ_CP041996:c4162870-4162583 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T134801 NZ_CP041996:c4162870-4162583 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT134801 WP_000063158.1 NZ_CP041996:c4162596-4162294 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT134801 NZ_CP041996:c4162596-4162294 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References