Detailed information of TA system TA134800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3665445..3666282
• Replicon: chromosome
• Accession: NZ_CP041996
• Organism: Escherichia coli strain AR Bank #0349

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A1M0D5U3
• Locus tag: FN958_RS17815
• Protein ID: WP_000227782.1
• Coordinates: 3665740..3666282 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: FN958_RS17810
• Protein ID: WP_001353405.1
• Coordinates: 3665445..3665756 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FN958_RS17785	3660465..3661412	+	948	WP_001239437.1	cytochrome o ubiquinol oxidase subunit II	-
FN958_RS17790	3661434..3663425	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FN958_RS17795	3663415..3664029	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FN958_RS17800	3664029..3664358	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FN958_RS17805	3664370..3665260	+	891	WP_000971327.1	heme o synthase	-
FN958_RS17810	3665445..3665756	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
FN958_RS17815	3665740..3666282	+	543	WP_000227782.1	GNAT family N-acetyltransferase	Toxin
FN958_RS17820	3666338..3667182	-	845	Protein_3459	sel1 repeat family protein	-
FN958_RS17825	3667589..3668953	+	1365	WP_001000978.1	MFS transporter	-
FN958_RS17830	3669081..3669572	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
FN958_RS17835	3669740..3670651	+	912	WP_000705841.1	2-dehydropantoate 2-reductase	-
FN958_RS17840	3670614..3671204	+	591	WP_001276320.1	protein deglycase YajL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19957.31 Da
• Isoelectric Point: 9.1763

>T134800 WP_000227782.1 NZ_CP041996:3665740-3666282 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T134800 NZ_CP041996:3665740-3666282 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTATTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT134800 WP_001353405.1 NZ_CP041996:3665445-3665756 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT134800 NZ_CP041996:3665445-3665756 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A1M0D5U3

Antitoxin

• AlphaFold DB: A0A829G7G3