Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3116..3705 Replicon plasmid unnamed1
Accession NZ_CP041966
Organism Xanthomonas citri pv. glycines strain K2

Toxin (Protein)


Gene name pemK Uniprot ID Q32X98
Locus tag FPL05_RS00020 Protein ID WP_012477455.1
Coordinates 3116..3448 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q32X99
Locus tag FPL05_RS00025 Protein ID WP_012477454.1
Coordinates 3448..3705 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPL05_RS00010 4..2181 + 2178 WP_012477457.1 relaxase/mobilization nuclease domain-containing protein -
FPL05_RS00015 2209..3027 + 819 WP_012477456.1 hypothetical protein -
FPL05_RS00020 3116..3448 - 333 WP_012477455.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FPL05_RS00025 3448..3705 - 258 WP_012477454.1 plasmid stable inheritance protein I Antitoxin
FPL05_RS00030 3971..4198 + 228 WP_012477437.1 hypothetical protein -
FPL05_RS00035 4195..4839 + 645 WP_012477436.1 recombinase family protein -
FPL05_RS00040 4906..5556 + 651 WP_012477435.1 ParA family protein -
FPL05_RS00045 5556..5810 + 255 WP_012477434.1 hypothetical protein -
FPL05_RS24195 6033..6191 + 159 WP_154666355.1 hypothetical protein -
FPL05_RS00050 6188..6992 + 805 Protein_10 trypsin-like peptidase domain-containing protein -
FPL05_RS00055 7058..7978 + 921 WP_012477431.1 RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..26496 26496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11581.24 Da        Isoelectric Point: 9.1684

>T134759 WP_012477455.1 NZ_CP041966:c3448-3116 [Xanthomonas citri pv. glycines]
MERGDIYLVSLDPTSGHEQQGTRPVLIVSPGPFNRLTKTPVVLPITTGGNFARTAGFTVSLTGGGTKTTGVVRCDQPRAL
DIGSRNGRKLESVSAAITDEVMAKLATILE

Download         Length: 333 bp

>T134759 NZ_CP041966:c3448-3116 [Xanthomonas citri pv. glycines]
ATGGAGCGGGGTGATATCTATCTTGTGTCTCTCGATCCTACTTCCGGCCATGAGCAACAGGGCACTCGCCCGGTCCTGAT
TGTGTCGCCGGGACCGTTCAACCGCCTTACCAAAACACCCGTGGTGCTACCGATCACCACGGGTGGCAATTTCGCCCGTA
CGGCAGGTTTCACGGTATCCCTCACGGGGGGAGGCACCAAAACGACCGGAGTCGTGCGCTGCGATCAACCGCGCGCCCTG
GACATTGGATCGCGTAACGGCCGCAAGCTGGAAAGCGTCTCAGCAGCGATCACCGATGAGGTGATGGCTAAGCTGGCGAC
CATACTTGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 8951.34 Da        Isoelectric Point: 5.5491

>AT134759 WP_012477454.1 NZ_CP041966:c3705-3448 [Xanthomonas citri pv. glycines]
MHTTNLRKVGGSVMLAVPPALLDVLHIGAGAKVGLAVDNGRLVVEPHARPRYTLADLLAQCDASAELASEDREWLDAKPV
GGELL

Download         Length: 258 bp

>AT134759 NZ_CP041966:c3705-3448 [Xanthomonas citri pv. glycines]
ATGCACACGACAAATCTGCGCAAGGTTGGCGGTTCGGTCATGCTCGCGGTTCCGCCTGCGCTGCTCGATGTGCTGCACAT
CGGGGCAGGGGCAAAAGTGGGCTTGGCTGTCGATAACGGCCGCTTGGTAGTCGAGCCACATGCGCGCCCGCGCTACACCC
TCGCCGACCTGCTCGCCCAATGCGATGCGTCAGCCGAGCTGGCTTCCGAAGATCGGGAATGGCTTGACGCCAAGCCTGTT
GGCGGCGAGCTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z6Z9Y5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9EPT3

References