Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 21808..22397 Replicon plasmid unnamed1
Accession NZ_CP041962
Organism Xanthomonas citri pv. glycines strain 1018

Toxin (Protein)


Gene name pemK Uniprot ID Q32X98
Locus tag FPL00_RS23215 Protein ID WP_012477455.1
Coordinates 21808..22140 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q32X99
Locus tag FPL00_RS23220 Protein ID WP_012477454.1
Coordinates 22140..22397 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPL00_RS23190 16991..17695 - 705 WP_007975110.1 hypothetical protein -
FPL00_RS23195 17685..18065 - 381 WP_176141440.1 hypothetical protein -
FPL00_RS23200 18392..18694 + 303 WP_012477458.1 hypothetical protein -
FPL00_RS23205 18696..20873 + 2178 WP_012477457.1 relaxase/mobilization nuclease domain-containing protein -
FPL00_RS23210 20901..21719 + 819 WP_012477456.1 hypothetical protein -
FPL00_RS23215 21808..22140 - 333 WP_012477455.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FPL00_RS23220 22140..22397 - 258 WP_012477454.1 plasmid stable inheritance protein I Antitoxin
FPL00_RS23225 22663..22890 + 228 WP_012477437.1 hypothetical protein -
FPL00_RS23230 22887..23531 + 645 WP_012477436.1 recombinase family protein -
FPL00_RS23235 23598..24248 + 651 WP_012477435.1 ParA family protein -
FPL00_RS23240 24248..24502 + 255 WP_012477434.1 hypothetical protein -
FPL00_RS23530 24725..24883 + 159 WP_154666355.1 hypothetical protein -
FPL00_RS23245 24880..25686 + 807 WP_016851361.1 serine protease -
FPL00_RS23250 25752..26672 + 921 WP_012477431.1 RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34066 34066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11581.24 Da        Isoelectric Point: 9.1684

>T134754 WP_012477455.1 NZ_CP041962:c22140-21808 [Xanthomonas citri pv. glycines]
MERGDIYLVSLDPTSGHEQQGTRPVLIVSPGPFNRLTKTPVVLPITTGGNFARTAGFTVSLTGGGTKTTGVVRCDQPRAL
DIGSRNGRKLESVSAAITDEVMAKLATILE

Download         Length: 333 bp

>T134754 NZ_CP041962:c22140-21808 [Xanthomonas citri pv. glycines]
ATGGAGCGGGGTGATATCTATCTTGTGTCTCTCGATCCTACTTCCGGCCATGAGCAACAGGGCACTCGCCCGGTCCTGAT
TGTGTCGCCGGGACCGTTCAACCGCCTTACCAAAACACCCGTGGTGCTACCGATCACCACGGGTGGCAATTTCGCCCGTA
CGGCAGGTTTCACGGTATCCCTCACGGGGGGAGGCACCAAAACGACCGGAGTCGTGCGCTGCGATCAACCGCGCGCCCTG
GACATTGGATCGCGTAACGGCCGCAAGCTGGAAAGCGTCTCAGCAGCGATCACCGATGAGGTGATGGCTAAGCTGGCGAC
CATACTTGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 8951.34 Da        Isoelectric Point: 5.5491

>AT134754 WP_012477454.1 NZ_CP041962:c22397-22140 [Xanthomonas citri pv. glycines]
MHTTNLRKVGGSVMLAVPPALLDVLHIGAGAKVGLAVDNGRLVVEPHARPRYTLADLLAQCDASAELASEDREWLDAKPV
GGELL

Download         Length: 258 bp

>AT134754 NZ_CP041962:c22397-22140 [Xanthomonas citri pv. glycines]
ATGCACACGACAAATCTGCGCAAGGTTGGCGGTTCGGTCATGCTCGCGGTTCCGCCTGCGCTGCTCGATGTGCTGCACAT
CGGGGCAGGGGCAAAAGTGGGCTTGGCTGTCGATAACGGCCGCTTGGTAGTCGAGCCACATGCGCGCCCGCGCTACACCC
TCGCCGACCTGCTCGCCCAATGCGATGCGTCAGCCGAGCTGGCTTCCGAAGATCGGGAATGGCTTGACGCCAAGCCTGTT
GGCGGCGAGCTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z6Z9Y5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9EPT3

References