Detailed information of TA system TA134750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 67035..67636
• Replicon: plasmid pEC2_5
• Accession: NZ_CP041960
• Organism: Escherichia coli strain EC2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: FOZ67_RS24875
• Protein ID: WP_001216045.1
• Coordinates: 67035..67415 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: FOZ67_RS24880
• Protein ID: WP_001190712.1
• Coordinates: 67415..67636 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOZ67_RS24850	62475..63959	-	1485	WP_000124150.1	terminase	-
FOZ67_RS24855	63959..65152	-	1194	WP_053903358.1	terminase	-
FOZ67_RS24860	65239..65691	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
FOZ67_RS24865	65780..66823	-	1044	WP_155367604.1	DUF968 domain-containing protein	-
FOZ67_RS24870	66851..67030	-	180	WP_000113019.1	hypothetical protein	-
FOZ67_RS24875	67035..67415	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FOZ67_RS24880	67415..67636	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FOZ67_RS24885	67709..68098	-	390	WP_000506726.1	DNA repair protein	-
FOZ67_RS24890	68222..68473	-	252	WP_001377386.1	DNA polymerase III subunit theta	-
FOZ67_RS25285	68923..69060	+	138	WP_000123562.1	hypothetical protein	-
FOZ67_RS24895	69135..69497	-	363	WP_001261544.1	hypothetical protein	-
FOZ67_RS24900	69494..70426	-	933	WP_077780118.1	hypothetical protein	-
FOZ67_RS24905	70408..70782	-	375	WP_023154376.1	hypothetical protein	-
FOZ67_RS24910	70789..71082	-	294	WP_001677496.1	hypothetical protein	-
FOZ67_RS24915	71261..71494	-	234	WP_000516537.1	hypothetical protein	-
FOZ67_RS24920	71577..72176	-	600	WP_053903357.1	DUF551 domain-containing protein	-
FOZ67_RS24925	72187..72450	-	264	WP_000224220.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98151	98151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T134750 WP_001216045.1 NZ_CP041960:c67415-67035 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T134750 NZ_CP041960:c67415-67035 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT134750 WP_001190712.1 NZ_CP041960:c67636-67415 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT134750 NZ_CP041960:c67636-67415 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6