Detailed information of TA system TA134727

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1841152..1841987
• Replicon: chromosome
• Accession: NZ_CP041955
• Organism: Escherichia coli strain EC2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: FOZ67_RS08685
• Protein ID: WP_000854761.1
• Coordinates: 1841610..1841987 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: FOZ67_RS08680
• Protein ID: WP_001295723.1
• Coordinates: 1841152..1841520 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOZ67_RS08655	1838267..1838425	+	159	WP_001323397.1	DUF905 family protein	-
FOZ67_RS08660	1838580..1839398	+	819	WP_001234729.1	DUF945 domain-containing protein	-
FOZ67_RS08665	1839740..1840213	+	474	WP_000855059.1	antirestriction protein	-
FOZ67_RS08670	1840229..1840705	+	477	WP_001186774.1	RadC family protein	-
FOZ67_RS08675	1840768..1840989	+	222	WP_000692345.1	DUF987 domain-containing protein	-
FOZ67_RS08680	1841152..1841520	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FOZ67_RS08685	1841610..1841987	+	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
FOZ67_RS08690	1841984..1842186	+	203	Protein_1697	hypothetical protein	-
FOZ67_RS08695	1842199..1842312	+	114	WP_001161660.1	DUF957 domain-containing protein	-
FOZ67_RS08700	1842743..1844283	-	1541	Protein_1699	IS66-like element ISEc22 family transposase	-
FOZ67_RS08705	1844332..1844679	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
FOZ67_RS08710	1844676..1845080	-	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
FOZ67_RS08715	1845574..1845963	+	390	WP_001445118.1	IS110 family transposase	-
FOZ67_RS08725	1846417..1846599	+	183	WP_001016348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T134727 WP_000854761.1 NZ_CP041955:1841610-1841987 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T134727 NZ_CP041955:1841610-1841987 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT134727 WP_001295723.1 NZ_CP041955:1841152-1841520 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT134727 NZ_CP041955:1841152-1841520 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52