Detailed information of TA system TA134721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 153864..154507
• Replicon: plasmid pKP2_3
• Accession: NZ_CP041949
• Organism: Klebsiella pneumoniae strain KP2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FOZ68_RS27665
• Protein ID: WP_014386165.1
• Coordinates: 154091..154507 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: FOZ68_RS27660
• Protein ID: WP_032408893.1
• Coordinates: 153864..154094 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOZ68_RS27635	148937..149932	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
FOZ68_RS27640	149939..151087	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
FOZ68_RS27645	151258..152415	-	1158	WP_032408895.1	acetylornithine deacetylase	-
FOZ68_RS27650	152431..153105	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
FOZ68_RS27655	153110..153544	-	435	WP_000103648.1	RidA family protein	-
FOZ68_RS27660	153864..154094	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FOZ68_RS27665	154091..154507	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
FOZ68_RS27670	154830..155798	+	969	WP_074422984.1	IS5 family transposase	-
FOZ68_RS27675	155978..156352	-	375	WP_032408891.1	hypothetical protein	-
FOZ68_RS27680	156408..156734	-	327	WP_004152639.1	hypothetical protein	-
FOZ68_RS27685	156731..157459	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
FOZ68_RS27690	157456..157887	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1	-	1..174723	174723
	-	flank	IS/Tn	-	-	154830..155798	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T134721 WP_014386165.1 NZ_CP041949:154091-154507 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T134721 NZ_CP041949:154091-154507 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT134721 WP_032408893.1 NZ_CP041949:153864-154094 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT134721 NZ_CP041949:153864-154094 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA