Detailed information of TA system TA134720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 129968..130713
• Replicon: plasmid pKP2_3
• Accession: NZ_CP041949
• Organism: Klebsiella pneumoniae strain KP2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: FOZ68_RS27535
• Protein ID: WP_032408901.1
• Coordinates: 130222..130713 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FOZ68_RS27530
• Protein ID: WP_014386183.1
• Coordinates: 129968..130234 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOZ68_RS27485	125520..125933	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
FOZ68_RS27490	125934..126212	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
FOZ68_RS27495	126202..126522	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FOZ68_RS27500	126603..126827	-	225	WP_014386189.1	hypothetical protein	-
FOZ68_RS27505	126838..127050	-	213	WP_014386188.1	hypothetical protein	-
FOZ68_RS27510	127112..127438	-	327	WP_014386187.1	hypothetical protein	-
FOZ68_RS27515	128075..128425	-	351	WP_014386186.1	hypothetical protein	-
FOZ68_RS28855	128422..128694	-	273	WP_032408902.1	hypothetical protein	-
FOZ68_RS27520	128884..129369	+	486	WP_014386185.1	hypothetical protein	-
FOZ68_RS27525	129613..129771	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
FOZ68_RS27530	129968..130234	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
FOZ68_RS27535	130222..130713	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
FOZ68_RS27540	131154..131405	-	252	WP_032408900.1	aldo/keto reductase	-
FOZ68_RS27545	131602..133194	-	1593	Protein_140	IS66 family transposase	-
FOZ68_RS27550	133225..133575	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
FOZ68_RS27555	133572..134012	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
FOZ68_RS27560	134274..135029	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1	-	1..174723	174723

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T134720 WP_032408901.1 NZ_CP041949:130222-130713 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T134720 NZ_CP041949:130222-130713 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT134720 WP_014386183.1 NZ_CP041949:129968-130234 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT134720 NZ_CP041949:129968-130234 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6