Detailed information of TA system TA134687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 379445..380051
• Replicon: chromosome
• Accession: NZ_CP041932
• Organism: Thermococcus aciditolerans strain SY113

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A100XW57
• Locus tag: FPV09_RS02010
• Protein ID: WP_058939638.1
• Coordinates: 379653..380051 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FPV09_RS02005
• Protein ID: WP_148882183.1
• Coordinates: 379445..379666 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV09_RS01975 (FPV09_01975)	374777..375061	-	285	WP_148882178.1	DUF167 domain-containing protein	-
FPV09_RS01980 (FPV09_01980)	375063..375563	-	501	WP_148882179.1	DUF402 domain-containing protein	-
FPV09_RS01985 (FPV09_01985)	375594..377306	-	1713	WP_148882180.1	glycine--tRNA ligase	-
FPV09_RS01990 (FPV09_01990)	377461..377691	+	231	WP_014011940.1	LSm family protein	-
FPV09_RS01995 (FPV09_01995)	377717..377905	+	189	WP_148882181.1	50S ribosomal protein L37e	-
FPV09_RS02000 (FPV09_02000)	377997..379397	+	1401	WP_148882182.1	MATE family efflux transporter	-
FPV09_RS02005 (FPV09_02005)	379445..379666	+	222	WP_148882183.1	hypothetical protein	Antitoxin
FPV09_RS02010 (FPV09_02010)	379653..380051	+	399	WP_058939638.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPV09_RS02015 (FPV09_02015)	380048..381142	-	1095	WP_148882184.1	VIT1/CCC1 transporter family protein	-
FPV09_RS02020 (FPV09_02020)	381191..382327	-	1137	WP_148882185.1	tRNA (guanine(10)-N(2))-dimethyltransferase	-
FPV09_RS02025 (FPV09_02025)	382374..382640	-	267	WP_148882186.1	50S ribosomal protein L35ae	-
FPV09_RS02030 (FPV09_02030)	382689..383348	-	660	WP_058939650.1	haloacid dehalogenase	-
FPV09_RS02035 (FPV09_02035)	383472..384518	+	1047	WP_148882187.1	Xaa-Pro dipeptidase PepQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (4-129)

Antitoxin

• Putative antitoxin VapB3-like (1-73)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14876.13 Da
• Isoelectric Point: 4.6810

>T134687 WP_058939638.1 NZ_CP041932:379653-380051 [Thermococcus aciditolerans]
VIAIDASVLAKYILLEPGWERVEELLFKDVVSLDYALAEVSNALWKHHVLYGEISREEFNKRSKVVDALPNVVVLESGMQ
YLEKSRGIAVNHRIPIYDALYIAQALKYGELATSDEKQGEIAEKLGVEVVYL

Nucleotide

• Length: 399 bp

>T134687 NZ_CP041932:379653-380051 [Thermococcus aciditolerans]
GTGATAGCAATTGACGCCTCCGTCCTTGCGAAGTACATTCTCCTTGAACCCGGTTGGGAACGGGTAGAGGAGCTACTTTT
TAAAGACGTCGTCTCACTTGACTATGCCTTGGCAGAGGTTTCAAACGCCCTCTGGAAACATCACGTCCTCTACGGTGAGA
TATCCCGGGAGGAGTTCAATAAGAGATCAAAGGTTGTGGATGCTCTCCCGAACGTCGTCGTTCTTGAGAGTGGAATGCAG
TATTTGGAGAAATCAAGGGGGATAGCTGTTAACCACCGAATCCCAATCTATGACGCCCTCTACATCGCTCAGGCCCTGAA
GTATGGCGAACTTGCCACCAGCGACGAAAAGCAGGGAGAAATAGCGGAGAAACTCGGCGTTGAGGTCGTCTACCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8578.85 Da
• Isoelectric Point: 5.8899

>AT134687 WP_148882183.1 NZ_CP041932:379445-379666 [Thermococcus aciditolerans]
MAVISVRIPDELKAKMKEYDINWSEEIRAFIVLRINEEEKRRTIEMMHELLAGGTPAKEGTAKRYVREDRDSN

Nucleotide

• Length: 222 bp

>AT134687 NZ_CP041932:379445-379666 [Thermococcus aciditolerans]
ATGGCTGTGATAAGCGTCCGTATTCCCGATGAACTAAAGGCCAAGATGAAGGAGTACGACATTAACTGGAGCGAGGAGAT
AAGGGCGTTCATAGTTTTGAGAATAAACGAAGAGGAGAAAAGAAGAACCATTGAGATGATGCACGAGCTGCTCGCTGGGG
GAACTCCTGCTAAAGAAGGAACCGCAAAAAGATACGTGAGGGAGGACCGTGATAGCAATTGA

Structures

Toxin

• AlphaFold DB: A0A100XW57