Detailed information of TA system TA134685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 127789..128432
• Replicon: plasmid pSECR18-2374A
• Accession: NZ_CP041928
• Organism: Klebsiella pneumoniae strain 18-2374

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FOM15_RS26870
• Protein ID: WP_014386165.1
• Coordinates: 127789..128205 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: FOM15_RS26875
• Protein ID: WP_032408893.1
• Coordinates: 128202..128432 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOM15_RS26845	124409..124840	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
FOM15_RS26850	124837..125565	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
FOM15_RS26855	125562..125888	+	327	WP_004152639.1	hypothetical protein	-
FOM15_RS26860	125944..126318	+	375	WP_032408891.1	hypothetical protein	-
FOM15_RS26865	126498..127466	-	969	WP_074422984.1	IS5 family transposase	-
FOM15_RS26870	127789..128205	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
FOM15_RS26875	128202..128432	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FOM15_RS26885	128752..129186	+	435	WP_000103648.1	RidA family protein	-
FOM15_RS26890	129191..129865	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
FOM15_RS26895	129881..131038	+	1158	WP_032408895.1	acetylornithine deacetylase	-
FOM15_RS26900	131209..132357	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
FOM15_RS26905	132364..133359	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	-	1..202623	202623
	-	flank	IS/Tn	-	-	126498..127466	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T134685 WP_014386165.1 NZ_CP041928:c128205-127789 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T134685 NZ_CP041928:c128205-127789 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT134685 WP_032408893.1 NZ_CP041928:c128432-128202 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT134685 NZ_CP041928:c128432-128202 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA