Detailed information of TA system TA134665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 47754..48255
• Replicon: plasmid unnamed1
• Accession: NZ_CP041926
• Organism: Klebsiella aerogenes strain Ka37751

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: FPV33_RS25350
• Protein ID: WP_000702247.1
• Coordinates: 47995..48255 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: FPV33_RS25345
• Protein ID: WP_001132407.1
• Coordinates: 47754..48005 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV33_RS25295	42811..43563	+	753	WP_063078685.1	conjugal transfer protein TraL	-
FPV33_RS25300	43565..44272	+	708	WP_021503228.1	hypothetical protein	-
FPV33_RS25305	44292..44618	+	327	WP_063078684.1	hypothetical protein	-
FPV33_RS25310	44749..45186	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
FPV33_RS25315	45211..45393	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
FPV33_RS25320	45951..46133	+	183	WP_001481829.1	hypothetical protein	-
FPV33_RS25325	46240..46506	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
FPV33_RS25330	46926..47204	-	279	WP_000545938.1	hypothetical protein	-
FPV33_RS25345	47754..48005	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
FPV33_RS25350	47995..48255	-	261	WP_000702247.1	hypothetical protein	Toxin
FPV33_RS25355	48307..48531	-	225	WP_000814381.1	hypothetical protein	-
FPV33_RS25360	48628..48837	-	210	WP_000866036.1	hypothetical protein	-
FPV33_RS25365	48879..49202	-	324	WP_000427403.1	hypothetical protein	-
FPV33_RS25375	49783..50112	-	330	WP_000855120.1	hypothetical protein	-
FPV33_RS25385	50871..52133	+	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1 / blaCTX-M-199	-	1..65845	65845
	-	flank	IS/Tn	blaCTX-M-199	-	50871..53261	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T134665 WP_000702247.1 NZ_CP041926:c48255-47995 [Klebsiella aerogenes]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T134665 NZ_CP041926:c48255-47995 [Klebsiella aerogenes]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT134665 WP_001132407.1 NZ_CP041926:c48005-47754 [Klebsiella aerogenes]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT134665 NZ_CP041926:c48005-47754 [Klebsiella aerogenes]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0