Detailed information of TA system TA134664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 2776..3298
• Replicon: plasmid unnamed1
• Accession: NZ_CP041926
• Organism: Klebsiella aerogenes strain Ka37751

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: FPV33_RS25080
• Protein ID: WP_000638823.1
• Coordinates: 3017..3298 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: FPV33_RS25075
• Protein ID: WP_000121741.1
• Coordinates: 2776..3027 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV33_RS25055	780..1418	+	639	Protein_1	conjugal transfer protein TrbJ	-
FPV33_RS25065	1686..1946	+	261	WP_144235218.1	EexN family lipoprotein	-
FPV33_RS25070	2044..2676	+	633	Protein_3	hypothetical protein	-
FPV33_RS25075	2776..3027	+	252	WP_000121741.1	hypothetical protein	Antitoxin
FPV33_RS25080	3017..3298	+	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPV33_RS25085	3366..3665	+	300	WP_000835764.1	IncN plasmid KikA protein	-
FPV33_RS25090	3668..4904	+	1237	Protein_7	TcpQ domain-containing protein	-
FPV33_RS25095	4910..5349	+	440	Protein_8	type IV pilus biogenesis protein PilM	-
FPV33_RS25105	5888..6472	+	585	Protein_9	lytic transglycosylase domain-containing protein	-
FPV33_RS25115	6834..7155	+	322	Protein_10	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1 / blaCTX-M-199	-	1..65845	65845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T134664 WP_000638823.1 NZ_CP041926:3017-3298 [Klebsiella aerogenes]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T134664 NZ_CP041926:3017-3298 [Klebsiella aerogenes]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT134664 WP_000121741.1 NZ_CP041926:2776-3027 [Klebsiella aerogenes]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT134664 NZ_CP041926:2776-3027 [Klebsiella aerogenes]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1