Detailed information of TA system TA134663

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4574239..4574942
• Replicon: chromosome
• Accession: NZ_CP041925
• Organism: Klebsiella aerogenes strain Ka37751

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: FPV33_RS22105
• Protein ID: WP_063445909.1
• Coordinates: 4574239..4574580 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: FPV33_RS22110
• Protein ID: WP_074167312.1
• Coordinates: 4574601..4574942 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV33_RS22085	4571309..4572028	+	720	WP_063445912.1	DNA/RNA non-specific endonuclease	-
FPV33_RS22090	4572144..4572488	+	345	WP_048229578.1	DUF1294 domain-containing protein	-
FPV33_RS22100	4572968..4573975	-	1008	WP_063445908.1	restriction endonuclease	-
FPV33_RS22105	4574239..4574580	-	342	WP_063445909.1	TA system toxin CbtA family protein	Toxin
FPV33_RS22110	4574601..4574942	-	342	WP_074167312.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FPV33_RS22115	4574953..4575495	-	543	WP_063445913.1	DNA repair protein RadC	-
FPV33_RS22120	4575508..4575948	-	441	WP_063445911.1	antirestriction protein	-
FPV33_RS22125	4575975..4576796	-	822	WP_143830422.1	DUF945 domain-containing protein	-
FPV33_RS22130	4576917..4577390	-	474	WP_063446170.1	hypothetical protein	-
FPV33_RS22135	4577461..4577913	-	453	WP_032728828.1	hypothetical protein	-
FPV33_RS22140	4577949..4578665	-	717	WP_063446171.1	WYL domain-containing protein	-
FPV33_RS22145	4578909..4579784	-	876	WP_063446172.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4572968..4597720	24752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12792.72 Da
• Isoelectric Point: 7.1652

>T134663 WP_063445909.1 NZ_CP041925:c4574580-4574239 [Klebsiella aerogenes]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYELVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T134663 NZ_CP041925:c4574580-4574239 [Klebsiella aerogenes]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGACTGACACTTAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTGAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12920.71 Da
• Isoelectric Point: 5.6811

>AT134663 WP_074167312.1 NZ_CP041925:c4574942-4574601 [Klebsiella aerogenes]
MKSTDLENDSFLKWGLNRDVTPCFGARLVQEGNRLYYLADRVSITGKFSNVEYLKLDEVFPRFISQMESMLTTGEMNPRL
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT134663 NZ_CP041925:c4574942-4574601 [Klebsiella aerogenes]
ATGAAATCTACTGACTTAGAGAACGATTCATTTCTCAAGTGGGGACTGAATCGTGATGTGACGCCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTGTACTATCTGGCTGACCGGGTCAGCATTACCGGTAAATTCAGTAACGTAGAATATT
TGAAATTGGATGAAGTATTCCCTCGTTTTATCAGCCAGATGGAATCGATGTTGACCACTGGTGAAATGAATCCTCGCCTT
GCCCACTGTGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA