Detailed information of TA system TA134661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2647768..2648507
• Replicon: chromosome
• Accession: NZ_CP041925
• Organism: Klebsiella aerogenes strain Ka37751

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FPV33_RS12790
• Protein ID: WP_063445746.1
• Coordinates: 2648022..2648507 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: FPV33_RS12785
• Protein ID: WP_015705366.1
• Coordinates: 2647768..2648034 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV33_RS12765	2644060..2644677	+	618	WP_045360840.1	response regulator transcription factor	-
FPV33_RS12770	2644773..2645270	+	498	WP_047080778.1	heme-binding protein	-
FPV33_RS12775	2645675..2646205	+	531	WP_045360836.1	N-acetyltransferase	-
FPV33_RS12780	2646260..2647495	-	1236	WP_015705367.1	MFS transporter	-
FPV33_RS12785	2647768..2648034	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
FPV33_RS12790	2648022..2648507	+	486	WP_063445746.1	GNAT family N-acetyltransferase	Toxin
FPV33_RS12795	2648579..2650195	+	1617	WP_042897505.1	FAD-NAD(P)-binding protein	-
FPV33_RS12800	2650314..2651414	+	1101	WP_063445747.1	NADH:flavin oxidoreductase/NADH oxidase	-
FPV33_RS12805	2651471..2651629	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
FPV33_RS12810	2651890..2652960	+	1071	WP_143830270.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17573.23 Da
• Isoelectric Point: 8.8793

>T134661 WP_063445746.1 NZ_CP041925:2648022-2648507 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEDSHQVVGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T134661 NZ_CP041925:2648022-2648507 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTAAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGATAGCC
ACCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCTTTTCACGGACAGGGGCTGGGGGCCGATCT
ACTCCATGATGCGCTGTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCTCTGACCG
ATAGCGCGAAACGATTCTATCTACACCACGGATTCAAAGCCTCAACCACCCAGGAACGCACACTTTTCCTCGCTTTGCCT
AAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT134661 WP_015705366.1 NZ_CP041925:2647768-2648034 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT134661 NZ_CP041925:2647768-2648034 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCGCGGACCGACTTTATCCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCACAATGGGAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9