Detailed information of TA system TA134650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 57125..57726
• Replicon: plasmid unnamed3
• Accession: NZ_CP041922
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1M0S1Q8
• Locus tag: FPV29_RS24510
• Protein ID: WP_024236459.1
• Coordinates: 57125..57505 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: FPV29_RS24515
• Protein ID: WP_001190712.1
• Coordinates: 57505..57726 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS24470	52608..52817	+	210	WP_000874156.1	hypothetical protein	-
FPV29_RS24475	52928..53779	+	852	WP_000611666.1	hypothetical protein	-
FPV29_RS24480	53812..54225	-	414	Protein_51	phage antirepressor KilAC domain-containing protein	-
FPV29_RS24495	55578..55781	-	204	Protein_53	phage antirepressor Ant	-
FPV29_RS24500	55870..56913	-	1044	WP_001561086.1	DUF968 domain-containing protein	-
FPV29_RS24505	56941..57120	-	180	WP_000113018.1	hypothetical protein	-
FPV29_RS24510	57125..57505	-	381	WP_024236459.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FPV29_RS24515	57505..57726	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPV29_RS24520	57799..58188	-	390	WP_000506727.1	DNA repair protein	-
FPV29_RS24525	58363..58935	+	573	WP_001133670.1	hypothetical protein	-
FPV29_RS24530	58942..59193	-	252	Protein_60	DNA polymerase III subunit theta	-
FPV29_RS24975	59643..59780	+	138	WP_000123562.1	hypothetical protein	-
FPV29_RS24540	59855..60217	-	363	WP_001261544.1	hypothetical protein	-
FPV29_RS24545	60214..60822	-	609	WP_077252779.1	hypothetical protein	-
FPV29_RS24550	61197..61529	-	333	WP_158212767.1	hypothetical protein	-
FPV29_RS24555	61534..61712	-	179	Protein_65	hypothetical protein	-
FPV29_RS24560	61714..62346	-	633	WP_144114370.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-55	-	1..96668	96668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13643.37 Da
• Isoelectric Point: 5.1514

>T134650 WP_024236459.1 NZ_CP041922:c57505-57125 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T134650 NZ_CP041922:c57505-57125 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT134650 WP_001190712.1 NZ_CP041922:c57726-57505 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT134650 NZ_CP041922:c57726-57505 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A1M0S1Q8

Antitoxin

• AlphaFold DB: A0A829CJB6