Detailed information of TA system TA134649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 46121..46622
• Replicon: plasmid unnamed2
• Accession: NZ_CP041921
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: FPV29_RS24095
• Protein ID: WP_000702247.1
• Coordinates: 46121..46381 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: FPV29_RS24100
• Protein ID: WP_001132407.1
• Coordinates: 46371..46622 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS24060	42243..43505	-	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
FPV29_RS24070	44264..44593	+	330	WP_000855120.1	hypothetical protein	-
FPV29_RS24080	45174..45497	+	324	WP_000427403.1	hypothetical protein	-
FPV29_RS24085	45539..45748	+	210	WP_000866036.1	hypothetical protein	-
FPV29_RS24090	45845..46069	+	225	WP_000814381.1	hypothetical protein	-
FPV29_RS24095	46121..46381	+	261	WP_000702247.1	hypothetical protein	Toxin
FPV29_RS24100	46371..46622	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
FPV29_RS24115	47172..47450	+	279	WP_000545938.1	hypothetical protein	-
FPV29_RS24120	47870..48136	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
FPV29_RS24125	48243..48425	-	183	WP_001481829.1	hypothetical protein	-
FPV29_RS24130	48983..49165	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
FPV29_RS24135	49190..49627	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
FPV29_RS24140	49758..50084	-	327	WP_063078684.1	hypothetical protein	-
FPV29_RS24145	50104..50811	-	708	WP_021503228.1	hypothetical protein	-
FPV29_RS24150	50813..51565	-	753	WP_063078685.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-199 / mcr-1.1	-	1..65845	65845
	-	flank	IS/Tn	blaCTX-M-199	-	41115..43505	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T134649 WP_000702247.1 NZ_CP041921:46121-46381 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T134649 NZ_CP041921:46121-46381 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT134649 WP_001132407.1 NZ_CP041921:46371-46622 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT134649 NZ_CP041921:46371-46622 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0