134648

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 40106..40599 Replicon plasmid unnamed2
Accession NZ_CP041921
Organism Escherichia coli strain Ec40743

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag FPV29_RS24040 Protein ID WP_001230709.1
Coordinates 40106..40360 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag FPV29_RS24045 Protein ID WP_000151456.1
Coordinates 40357..40599 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPV29_RS24010 36414..36575 + 162 WP_001434039.1 type I toxin-antitoxin system Hok family toxin -
FPV29_RS24015 36724..37320 + 597 WP_000125165.1 hypothetical protein -
FPV29_RS24960 37338..37622 - 285 WP_001162371.1 hypothetical protein -
FPV29_RS24025 37696..38361 - 666 WP_015387337.1 P-loop NTPase -
FPV29_RS24965 38598..38951 + 354 WP_157806643.1 hypothetical protein -
FPV29_RS24030 38948..39463 + 516 WP_001025393.1 J domain-containing protein -
FPV29_RS24035 39567..40109 + 543 WP_001083904.1 hypothetical protein -
FPV29_RS24040 40106..40360 + 255 WP_001230709.1 hypothetical protein Toxin
FPV29_RS24045 40357..40599 + 243 WP_000151456.1 hypothetical protein Antitoxin
FPV29_RS24050 40736..41068 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
FPV29_RS24055 41115..41990 - 876 WP_087587947.1 class A extended-spectrum beta-lactamase CTX-M-199 -
FPV29_RS24060 42243..43505 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
FPV29_RS24070 44264..44593 + 330 WP_000855120.1 hypothetical protein -
FPV29_RS24080 45174..45497 + 324 WP_000427403.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-199 / mcr-1.1 - 1..65845 65845
- flank IS/Tn blaCTX-M-199 - 41115..43505 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(8-48)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T134648 WP_001230709.1 NZ_CP041921:40106-40360 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T134648 NZ_CP041921:40106-40360 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT134648 WP_000151456.1 NZ_CP041921:40357-40599 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT134648 NZ_CP041921:40357-40599 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References