Detailed information of TA system TA134631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3513060..3513897
• Replicon: chromosome
• Accession: NZ_CP041919
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FPV29_RS17145
• Protein ID: WP_000227784.1
• Coordinates: 3513355..3513897 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A3T7ZCX8
• Locus tag: FPV29_RS17140
• Protein ID: WP_001442339.1
• Coordinates: 3513060..3513371 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS17115	3508080..3509027	+	948	WP_001239444.1	cytochrome o ubiquinol oxidase subunit II	-
FPV29_RS17120	3509049..3511040	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FPV29_RS17125	3511030..3511644	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FPV29_RS17130	3511644..3511973	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FPV29_RS17135	3511985..3512875	+	891	WP_000971336.1	heme o synthase	-
FPV29_RS17140	3513060..3513371	+	312	WP_001442339.1	DUF1778 domain-containing protein	Antitoxin
FPV29_RS17145	3513355..3513897	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FPV29_RS17150	3513953..3514888	-	936	WP_001297127.1	sel1 repeat family protein	-
FPV29_RS17155	3515296..3516660	+	1365	WP_001000983.1	MFS transporter	-
FPV29_RS17160	3516788..3517279	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
FPV29_RS17165	3517447..3518358	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T134631 WP_000227784.1 NZ_CP041919:3513355-3513897 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T134631 NZ_CP041919:3513355-3513897 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT134631 WP_001442339.1 NZ_CP041919:3513060-3513371 [Escherichia coli]
MPGKTATLPDVDKTLKNARVEIKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT134631 NZ_CP041919:3513060-3513371 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAAATCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A3T7ZCX8