Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3478983..3479601 Replicon chromosome
Accession NZ_CP041919
Organism Escherichia coli strain Ec40743

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FPV29_RS16975 Protein ID WP_001291435.1
Coordinates 3479383..3479601 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FPV29_RS16970 Protein ID WP_000344800.1
Coordinates 3478983..3479357 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPV29_RS16960 3474072..3475265 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FPV29_RS16965 3475288..3478437 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FPV29_RS16970 3478983..3479357 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FPV29_RS16975 3479383..3479601 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FPV29_RS16980 3479773..3480324 + 552 WP_000102564.1 maltose O-acetyltransferase -
FPV29_RS16985 3480440..3480910 + 471 WP_000136192.1 YlaC family protein -
FPV29_RS16990 3481074..3482624 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FPV29_RS16995 3482666..3483019 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FPV29_RS17005 3483398..3483709 + 312 WP_000409911.1 MGMT family protein -
FPV29_RS17010 3483740..3484312 - 573 WP_023156216.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T134630 WP_001291435.1 NZ_CP041919:3479383-3479601 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T134630 NZ_CP041919:3479383-3479601 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT134630 WP_000344800.1 NZ_CP041919:3478983-3479357 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT134630 NZ_CP041919:3478983-3479357 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References