Detailed information of TA system TA134620

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1816313..1817148
• Replicon: chromosome
• Accession: NZ_CP041919
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: FPV29_RS08830
• Protein ID: WP_000854752.1
• Coordinates: 1816313..1816690 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: FPV29_RS08835
• Protein ID: WP_001278232.1
• Coordinates: 1816780..1817148 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS08790	1811833..1812306	+	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-
FPV29_RS08795	1812504..1813562	+	1059	WP_001200892.1	FUSC family protein	-
FPV29_RS08800	1813734..1814063	+	330	WP_000450409.1	DUF496 family protein	-
FPV29_RS08805	1814164..1814346	-	183	WP_024167529.1	ethanolamine utilization protein	-
FPV29_RS08815	1814800..1815828	-	1029	Protein_1694	IS110 family transposase	-
FPV29_RS08820	1815987..1816100	-	114	WP_001161660.1	DUF957 domain-containing protein	-
FPV29_RS08825	1816113..1816316	-	204	WP_000761705.1	hypothetical protein	-
FPV29_RS08830	1816313..1816690	-	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
FPV29_RS08835	1816780..1817148	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FPV29_RS08840	1817311..1817532	-	222	WP_000692345.1	DUF987 domain-containing protein	-
FPV29_RS08845	1817595..1818071	-	477	WP_001186725.1	RadC family protein	-
FPV29_RS08850	1818087..1818572	-	486	WP_021533007.1	antirestriction protein	-
FPV29_RS08855	1818627..1819445	-	819	WP_001234620.1	DUF945 domain-containing protein	-
FPV29_RS08865	1819545..1819778	-	234	WP_001119729.1	DUF905 family protein	-
FPV29_RS08870	1819857..1820312	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T134620 WP_000854752.1 NZ_CP041919:c1816690-1816313 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T134620 NZ_CP041919:c1816690-1816313 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT134620 WP_001278232.1 NZ_CP041919:c1817148-1816780 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT134620 NZ_CP041919:c1817148-1816780 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72