Detailed information of TA system TA134618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1305572..1306197
• Replicon: chromosome
• Accession: NZ_CP041919
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8S7SZU1
• Locus tag: FPV29_RS06375
• Protein ID: WP_000911327.1
• Coordinates: 1305799..1306197 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FPV29_RS06370
• Protein ID: WP_000450524.1
• Coordinates: 1305572..1305799 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS06345	1301374..1301844	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FPV29_RS06350	1301844..1302416	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FPV29_RS06355	1302562..1303440	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPV29_RS06360	1303457..1304491	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FPV29_RS06365	1304704..1305417	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FPV29_RS06370	1305572..1305799	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FPV29_RS06375	1305799..1306197	+	399	WP_000911327.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FPV29_RS06380	1306344..1307207	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
FPV29_RS06385	1307222..1309237	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
FPV29_RS06390	1309311..1310009	+	699	WP_000679823.1	esterase	-
FPV29_RS06395	1310119..1310319	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T134618 WP_000911327.1 NZ_CP041919:1305799-1306197 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMEIIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T134618 NZ_CP041919:1305799-1306197 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGATTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT134618 WP_000450524.1 NZ_CP041919:1305572-1305799 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT134618 NZ_CP041919:1305572-1305799 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33