Detailed information of TA system TA134614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 262580..263211
• Replicon: chromosome
• Accession: NZ_CP041919
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: F4NNK6
• Locus tag: FPV29_RS01210
• Protein ID: WP_001259388.1
• Coordinates: 262580..262855 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8S7TCM0
• Locus tag: FPV29_RS01215
• Protein ID: WP_000593556.1
• Coordinates: 262852..263211 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS01195	257584..258651	+	1068	WP_000361485.1	HlyD family secretion protein	-
FPV29_RS01200	258648..261383	+	2736	WP_000149125.1	ribosome-associated ATPase/putative transporter RbbA	-
FPV29_RS01205	261383..262507	+	1125	WP_001314210.1	ABC transporter permease	-
FPV29_RS01210	262580..262855	+	276	WP_001259388.1	type II toxin-antitoxin system HicA family toxin	Toxin
FPV29_RS01215	262852..263211	+	360	WP_000593556.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FPV29_RS01220	263331..263732	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
FPV29_RS01225	263738..264544	-	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
FPV29_RS01230	264541..265305	-	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
FPV29_RS01235	265305..266138	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
FPV29_RS01240	266135..267079	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10151.89 Da
• Isoelectric Point: 10.7076

>T134614 WP_001259388.1 NZ_CP041919:262580-262855 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T134614 NZ_CP041919:262580-262855 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.1987

>AT134614 WP_000593556.1 NZ_CP041919:262852-263211 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHV

Nucleotide

• Length: 360 bp

>AT134614 NZ_CP041919:262852-263211 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FFA2