Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3069155..3069834 Replicon chromosome
Accession NZ_CP041876
Organism Mycobacterium tuberculosis strain 1-0006P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag FPJ30_RS14435 Protein ID WP_003414059.1
Coordinates 3069155..3069571 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FPJ30_RS14440 Protein ID WP_003414061.1
Coordinates 3069568..3069834 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ30_RS14415 3065207..3066109 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
FPJ30_RS14420 3066178..3066930 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FPJ30_RS14425 3067174..3067449 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
FPJ30_RS14430 3067446..3069068 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
FPJ30_RS14435 3069155..3069571 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
FPJ30_RS14440 3069568..3069834 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ30_RS14445 3069860..3070255 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
FPJ30_RS14450 3070252..3070521 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ30_RS14455 3070531..3071625 - 1095 WP_003414068.1 restriction endonuclease subunit S -
FPJ30_RS14460 3071622..3072041 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FPJ30_RS14465 3072115..3072594 - 480 WP_003414073.1 dihydrofolate reductase -
FPJ30_RS14470 3072665..3073453 - 789 WP_193077369.1 thymidylate synthase -
FPJ30_RS14475 3073609..3074346 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T134602 WP_003414059.1 NZ_CP041876:c3069571-3069155 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T134602 NZ_CP041876:c3069571-3069155 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT134602 WP_003414061.1 NZ_CP041876:c3069834-3069568 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT134602 NZ_CP041876:c3069834-3069568 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References