Detailed information of TA system TA134546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3160595..3161199
• Replicon: chromosome
• Accession: NZ_CP041875
• Organism: Mycobacterium tuberculosis strain 1-0007P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FPJ31_RS14930
• Protein ID: WP_003414492.1
• Coordinates: 3160595..3160987 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FPJ31_RS14935
• Protein ID: WP_003414495.1
• Coordinates: 3160984..3161199 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ31_RS14900	3155745..3156533	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FPJ31_RS14905	3156867..3157412	-	546	WP_014584866.1	DUF1802 family protein	-
FPJ31_RS14910	3157684..3158568	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FPJ31_RS14915	3158571..3159458	-	888	WP_015630563.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FPJ31_RS14920	3159763..3160308	-	546	WP_019284034.1	DUF1802 family protein	-
FPJ31_RS14925	3160305..3160574	-	270	WP_003414489.1	DUF2277 family protein	-
FPJ31_RS14930	3160595..3160987	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ31_RS14935	3160984..3161199	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FPJ31_RS14940	3161246..3161995	+	750	WP_003414497.1	enoyl-CoA hydratase	-
FPJ31_RS14945	3162074..3163156	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
FPJ31_RS14950	3163149..3164459	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
FPJ31_RS14955	3164462..3165289	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FPJ31_RS14960	3165286..3166197	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3140400..3162452	22052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T134546 WP_003414492.1 NZ_CP041875:c3160987-3160595 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T134546 NZ_CP041875:c3160987-3160595 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT134546 WP_003414495.1 NZ_CP041875:c3161199-3160984 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT134546 NZ_CP041875:c3161199-3160984 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8