Detailed information of TA system TA134542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3092586..3093265
• Replicon: chromosome
• Accession: NZ_CP041875
• Organism: Mycobacterium tuberculosis strain 1-0007P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPJ31_RS14555
• Protein ID: WP_003414059.1
• Coordinates: 3092586..3093002 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ31_RS14560
• Protein ID: WP_003414061.1
• Coordinates: 3092999..3093265 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ31_RS14535	3088638..3089540	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ31_RS14540	3089609..3090361	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ31_RS14545	3090605..3090880	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ31_RS14550	3090877..3092499	-	1623	WP_015630537.1	type I restriction-modification system subunit M	-
FPJ31_RS14555	3092586..3093002	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPJ31_RS14560	3092999..3093265	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ31_RS14565	3093291..3093686	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPJ31_RS14570	3093683..3093952	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ31_RS14575	3093962..3095056	-	1095	WP_015630539.1	restriction endonuclease subunit S	-
FPJ31_RS14580	3095053..3095472	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ31_RS14585	3095546..3096025	-	480	WP_003414073.1	dihydrofolate reductase	-
FPJ31_RS14590	3096096..3096896	-	801	WP_193070276.1	thymidylate synthase	-
FPJ31_RS14595	3097052..3097789	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T134542 WP_003414059.1 NZ_CP041875:c3093002-3092586 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T134542 NZ_CP041875:c3093002-3092586 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT134542 WP_003414061.1 NZ_CP041875:c3093265-3092999 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT134542 NZ_CP041875:c3093265-3092999 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64