Detailed information of TA system TA134478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2866289..2866920
• Replicon: chromosome
• Accession: NZ_CP041874
• Organism: Mycobacterium tuberculosis strain 1-0009P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ32_RS13345
• Protein ID: WP_003413174.1
• Coordinates: 2866543..2866920 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ32_RS13340
• Protein ID: WP_003413167.1
• Coordinates: 2866289..2866546 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ32_RS13310	2862204..2862518	+	315	WP_009937839.1	hypothetical protein	-
FPJ32_RS13315	2862814..2864025	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ32_RS13320	2864152..2864811	+	660	WP_003902296.1	hypothetical protein	-
FPJ32_RS13325	2864808..2865470	+	663	WP_003905878.1	hypothetical protein	-
FPJ32_RS13330	2865467..2865744	+	278	Protein_2631	type II toxin-antitoxin system VapB family antitoxin	-
FPJ32_RS13335	2865837..2866250	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ32_RS13340	2866289..2866546	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ32_RS13345	2866543..2866920	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ32_RS13350	2866936..2867310	-	375	WP_003413177.1	hypothetical protein	-
FPJ32_RS13355	2867410..2867805	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ32_RS13360	2867802..2868047	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ32_RS13365	2868458..2868877	-	420	WP_003413190.1	A24 family peptidase	-
FPJ32_RS13370	2868889..2869698	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ32_RS13375	2869695..2870948	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ32_RS13380	2870941..2871453	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T134478 WP_003413174.1 NZ_CP041874:2866543-2866920 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T134478 NZ_CP041874:2866543-2866920 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT134478 WP_003413167.1 NZ_CP041874:2866289-2866546 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT134478 NZ_CP041874:2866289-2866546 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5