Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3147322..3147926 Replicon chromosome
Accession NZ_CP041871
Organism Mycobacterium tuberculosis strain 1-0021P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag FPJ35_RS14875 Protein ID WP_003414492.1
Coordinates 3147322..3147714 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag FPJ35_RS14880 Protein ID WP_003414495.1
Coordinates 3147711..3147926 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ35_RS14845 3142472..3143260 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
FPJ35_RS14850 3143594..3144139 - 546 WP_014584866.1 DUF1802 family protein -
FPJ35_RS14855 3144411..3145295 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
FPJ35_RS14860 3145298..3146185 - 888 WP_015630563.1 type IV toxin-antitoxin system AbiEi family antitoxin -
FPJ35_RS14865 3146490..3147035 - 546 WP_019284034.1 DUF1802 family protein -
FPJ35_RS14870 3147032..3147301 - 270 WP_003414489.1 DUF2277 family protein -
FPJ35_RS14875 3147322..3147714 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
FPJ35_RS14880 3147711..3147926 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FPJ35_RS14885 3147973..3148722 + 750 WP_003414497.1 enoyl-CoA hydratase -
FPJ35_RS14890 3148801..3149883 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
FPJ35_RS14895 3149876..3151186 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
FPJ35_RS14900 3151189..3152016 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
FPJ35_RS14905 3152013..3152924 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T134306 WP_003414492.1 NZ_CP041871:c3147714-3147322 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T134306 NZ_CP041871:c3147714-3147322 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT134306 WP_003414495.1 NZ_CP041871:c3147926-3147711 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT134306 NZ_CP041871:c3147926-3147711 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References